牙周炎对动脉粥样硬化影响的动物实验研究  

An animal experiment study on the effect of periodontitis on atherosclerosis

作　　者：王洲[1] 张明珠[1] 喻卓[2] 税艳青[1] 丁芸[1] 雷雅燕[1] 

机构地区：[1]昆明医学院附属口腔医院口腔内科 [2]昆明医学院第一附属医院心内科,昆明650031

出　　处：《华西口腔医学杂志》2012年第3期308-313,共6页West China Journal of Stomatology

基　　金：云南省科技厅省校联合专项基金资助项目(2008CD057);云南省科技厅科技计划基金资助项目(2010ZC115)

摘　　要：目的建立牙周炎和动脉粥样硬化(AS)动物复合模型,探讨牙周炎对AS的影响。方法 36只日本大耳白兔随机分为4组,包括单纯牙周炎(CP)组、牙周炎合并AS(CP+AS)组、单纯AS组和对照组,采用结扎结合涂牙龈卟啉单胞菌(P.gingivalis)的方法建立牙周炎动物模型,单侧髂动脉球囊内皮损伤术建立AS模型。建模成功后,采用苏木精-伊红(HE)染色观察样本的组织病理学改变;Elastica van Gieson(EVG)弹力纤维染色进行形态分析,计算髂动脉横断面内膜与中膜面积的比值。采用巢氏聚合酶链反应(PCR)法检测受损动脉壁中P.gingivalis 16S rDNA。酶联免疫吸附测定(ELISA)法检测系统炎性因子的表达,包括C反应蛋白(CRP)、白细胞介素-6(IL-6)和白细胞介素-1β(IL-1β)。结果 CP+AS组的CRP、IL-6及IL-1β表达水平较其他组明显升高(P<0.01),血管的内膜与中膜面积比均较其余组大(P<0.01)。在CP和CP+AS组的髂动脉中检出有P.gingivalis 16S rDNA存在。结论牙周炎对患AS的动脉内膜的增厚可能起促进作用,其作用主要通过系统炎性因子的上调和细菌局部感染的作用来实现。Objective To make an animal periodontitis and atherosclerosis compound model,and to study the effects of periodontitis on atherosclerosis.Methods 36 Japan rabbits were randomly divided into four groups： Including perio-dontitis model group,periodontitis and atherosclerosis compound model group,atherosclerosis model group and control group.Periodontitis model was initiated by ligating floss around teeth cervical and oral inoculating with Porphyromonas gingivalis（P.gingivalis）.Atherosclerosis was established by single iliac artery of balloon-injured rabbit.Histopathological change of injured iliac artery was observed under optical microscope after hematoxylin-eosion stain.Elastica van Giesonstained sections were used for the morphometric analysis.We measured intimal and medial lesion areas in iliac artery cross-sections as well as the intimal/medial ratio（I/M）.We also analyzed P.gingivalis 16S rDNA amplification with nested-polymerase chain reaction（nested-PCR）,and detect systemic proinflammatory mediators with enzyme linked immunosorbent assay（ELISA）,including C-reactive protein（CRP）,interleukin-6（IL-6） and interleukin-1β（IL-1β）.Results The serum levels of CRP,IL-6 and IL-1β increased obviously among the compound model group than other groups（P0.01）.Histopathological observation revealed the compound model group in I/M was bigger than other groups（P 0.01）.P.gingivalis 16S rDNA was detected among the periodontitis model group and the compound model group by nested-PCR.Conclusion Periodontitis may accelerate intimal hyperplasia in balloon-injured iliac arteries by upgrade of systemic inflammation factors and local bacterial infection.

关 键 词：牙周炎 动脉粥样硬化 C反应蛋白 白细胞介素-6 白细胞介素-1Β 

分 类 号：R781.4[医药卫生—口腔医学]