人前列腺癌细胞系DU 145中肿瘤干细胞样侧群细胞的分离  

Identification of cancer stem cell-like side population cells in human prostate cancer cell line DU 145

作　　者：殷波[1] 刘岗[1] 张辉[1] 李明[1] 隋文印[1] 宋永胜[1] 

机构地区：[1]中国医科大学附属盛京医院泌尿外科,辽宁沈阳110004

出　　处：《现代肿瘤医学》2012年第6期1110-1112,共3页Journal of Modern Oncology

基　　金：辽宁省教育厅高等学校科研基金项目(编号:2009A735)

摘　　要：目的:分离人前列腺癌细胞系DU 145中的侧群(side population,SP)细胞,并初步分析其生物学特性。方法:采用荧光激活细胞分类(fluorescence activated cell sorting,FACS)技术,从DU 145细胞中分离出侧群细胞,并检测其比例;继而培养于无血清培养基中,观察其生长特性。采用反转录聚合酶链反应(RT-PCR)技术检测侧群细胞中ABCG2的表达水平。结果:DU 1 4 5细胞中存在含量极少的侧群细胞,比例约1.1%;培养于无血清培养基中成簇生长。和对应的母系DU 145细胞相比,DU 145侧群细胞的ABCG2表达增高。结论:人前列腺癌细胞系DU 145中存在具有肿瘤干细胞特性的侧群细胞。Objective：To identify the side population （SP） cells from human prostate cancer cell line DU 145 and characterize DU 145 SP cells preliminary. Methods： Fluorescence activated cell sorting （FACS） was applied to iso- late SP cells from DU 145 cell line. The isolated SP cells were cultured in serum - free medium to observe the growth pattern. Reverse transcription - polymerase chain reaction （ RT - PCR） was used to detect the expression of ABCG2 in DU 145 SP cells and parental DU 145 cells. Results： SP cells were isolated from DU 145 cells by flow cytometry, with a proportion of 1.1%. When cultured in serum - free medium, SP cells showed a clustered growth pattern com- pared to the parental DU 145 cells. Furthermore, a higher expression of ABCG2 in SP cells was detected by RT - PCR in contrast to parental DU 145 cells. Conclusion： DU 145 cell line contained SP cells with cancer stem cell properties.

关 键 词：前列腺癌 侧群细胞 肿瘤干细胞 ABCG2 

分 类 号：R73-35[医药卫生—肿瘤] R737.25[医药卫生—临床医学]