nm23-H1转染对肺癌L9981细胞株整合素家族的影响  

Influence of nm23-H1 tansfection on expression of integrins in lung cancer cell

作　　者：郑海霞[1] 陆红[2] 申东兰[1] 彭安[1] 何艳玲[1] 曹菊秀[1] 

机构地区：[1]北京大学深圳医院,广东深圳518036 [2]新疆医科大学第五附属医院,新疆乌鲁木齐830011

出　　处：《现代肿瘤医学》2012年第6期1117-1119,共3页Journal of Modern Oncology

基　　金：国家自然科学基金资助项目(编号:30070333)

摘　　要：目的:探讨nm23-H1基因对肺癌细胞整合素分子表达的影响。方法:利用半定量RT-PCR技术检测转染前后integrinβ1、integrinβ3基因的mRNA表达;流式细胞仪检测转染前后integrinβ1、integrinβ3基因的蛋白表达。结果:转染后细胞株integrinβ1、integrinβ3 mRNA与蛋白表达下调。结论:nm23-H1基因对肺癌细胞株L9981 integrinβ1、integrinβ3 mRNA与蛋白表达具有负调控作用。Objective：To study the infection of nm23-H1 gene on the expression of integrins in lung cancer cell.Methods： Semi-RT-PCR was used to detect the difference mRNA expression of integrinβ1 and integrinβ3 betwteen transfected and non-transfected cell lines;Flow cytometry was used to detect the difference protein expression of integrinβ1 and integrinβ3 betwteen transfected and non-transfected cell lines.Results： The expression of mRNA and protein of integrinβ1 and integrinβ3 of transfected cell line was weaker than that of non-transfected cell line.Conclusion： nm23-H1 can down-regulate expression of integrins and thus inhibit the metastasis of lung cancer cell.

关 键 词：NM23-H1 肺癌细胞 整合素 肿瘤转移 

分 类 号：R730[医药卫生—肿瘤] R734.2[医药卫生—临床医学]