雷帕霉素联合多西紫杉醇诱导肺癌95D细胞凋亡的机制研究  

Effects of rapamycin and docetaxel on apoptosis of 95D lung cancer cells

作　　者：牛慧彦[1] 姜洪芳[1] 王佳贺[1] 阚亮[1] 何平[1] 

机构地区：[1]中国医科大学附属盛京医院老年病科,辽宁沈阳110004

出　　处：《现代肿瘤医学》2012年第6期1123-1125,共3页Journal of Modern Oncology

基　　金：辽宁省博士科研启动基金资助项目(编号:20111096);辽宁省科技计划项目(编号:2009225008-10)

摘　　要：目的:探讨哺乳动物雷帕霉素靶蛋白信号通路抑制剂雷帕霉素(rapamycin,Rapa)联合多西紫杉醇(docetaxel,DTX)对肺癌95D细胞系凋亡的影响。方法:流式细胞仪检测雷帕霉素及联合多西紫杉醇对95D细胞凋亡的影响,RT-PCR方法和Western blot检测凋亡基因survivin mRNA和蛋白的表达。结果:mTOR抑制剂雷帕霉素和多西紫杉醇单独处理组均能促进肺癌细胞的凋亡,下调凋亡基因survivin mRNA和蛋白的表达;联合应用雷帕霉素和多西紫杉醇促凋亡作用更强,survivin mRNA和蛋白的表达下降更明显。结论:雷帕霉素联合多西紫杉可以降低survivin的表达,从而起到促进肿瘤细胞凋亡的作用,为临床寻找新型抗肿瘤药物及治疗肺癌的新化学治疗方案提供了理论和实践基础。Objective：To study the effects of rapamycin and docetaxel on apoptosis of 95D lung cancer cell lines.Methods： Lung cancer cells were treated with docetaxel and rapamycin.The effect on apoptosis was measured by flow cytometry.Survivin mRNA expression was detected by RT-PCR method,and protein expression was detected by Western blot method.Results： The cell apoptosis rate in combination group was higher than that in control,Rapa and DTX groups（P〈0.05）.The expressions of survivin mRNA and protein in combination group were lower than those in control,Rapa and DTX groups（P〈0.05）.Conclusion： Apoptosis-inducing effects of rapamycin and docetatxel may be exerted through down-regulating survivin expression.Our results suggest that a therapeutic strategy combining specific inhibitor of mTOR with docetaxel may be a promising approach to an improved treatment of advanced lung cancer.

关 键 词：雷帕霉素 多西紫杉醇 肺癌 凋亡 SURVIVIN 

分 类 号：R73-361[医药卫生—肿瘤] R734.2[医药卫生—临床医学]