左旋棉酚对裸鼠人前列腺癌PC-3细胞皮下移植瘤的生长抑制作用  

Inhibitory effect of(-)-gossypol on human prostate cancer PC-3 cells subcutaneous xenograft in nude mice

作　　者：顾顺利[1] 黄晓峰[2] 穆士杰[1] 胡兴斌[1] 安群星[1] 刘志新[1] 张献清[1] 

机构地区：[1]第四军医大学西京医院输血科,陕西西安710032 [2]第四军医大学中心实验室,陕西西安710032

出　　处：《现代肿瘤医学》2012年第6期1141-1145,共5页Journal of Modern Oncology

摘　　要：目的:探讨左旋棉酚对裸鼠人前列腺癌PC-3细胞皮下移植瘤的生长抑制作用及其机制。方法:建立裸鼠人前列腺癌PC-3细胞皮下移植瘤模型,将移植瘤裸鼠80只随机分成4组,每组20只,分别为10.0mg/kg、5.0mg/kg、2.5mg/kg左旋棉酚治疗组和对照组,进行疗效分析与组织病理形态学观察,同时检测肿瘤组织内PCNA、bcl-2、caspase-3和caspase-8的表达。结果:左旋棉酚可使人前列腺癌PC-3细胞荷瘤裸鼠存活率提高,肿瘤体积缩小,肿瘤组织坏死明显,PCNA与bcl-2表达减少,caspase-3和caspase-8表达增加,但高剂量左旋棉酚对PC-3荷瘤裸鼠肝脏和肠道有一定毒性。结论:当治疗剂量大于5.0mg/kg时,左旋棉酚可通过增殖抑制和诱导凋亡,明显抑制裸鼠人前列腺癌PC-3细胞皮下移植瘤的生长。Objective：To investigate the inhibitory effect and its mechanisms of（-）-gossypol on prostate cancer PC-3 cells subcutaneous xenograft in nude mice.Methods： Nude mice xenograft models were established with PC-3 cells,eighty mice were randomly divided into 4 groups,including 10.0mg/kg,5.0mg/kg and 2.5mg/kg therapy groups and control group,twenty mice in each group.The therapeutic effect and tissue pathology were observed;The expression of PCNA,bcl-2,caspase-3 and caspase-8 were detected.Results：（-）-Gossypol can increase the survival rate of PC-3-breaing nude mice and reduce the tumor volume.The necrosis of tumor tissues was obvious after（-）-gossypol treatment.The expression of PCNA and bcl-2 in tumor tissues were decreased,but the expression of caspase-3 and caspase-8 were increased.（-）-Gossypol can cause the toxicity on the liver and intestinal tract at higher dosage.Conclusion：（-）-Gossypol can inhibit the growth of xenograft by inducing apoptosis and proliferation inhibition when the therapy dosage greater than 5.0mg/kg.

关 键 词：前列腺癌 左旋棉酚 PC-3细胞 移植瘤 裸鼠 

分 类 号：R730.3[医药卫生—肿瘤]