蓖麻种质资源多样性AP-PCR与RMAPD分析  

Castor Germplasm Diversity Analysis Using AP-PCR and RMAPD

作　　者：何东[1] 王昌禄[1] 李伟[1] 杨朝晖[1] 杨桂仙[1] 杨华[1] 王玉荣[1] 陈勉华[1] 李风娟[1] 叶锋[2] Grace.Chen 

机构地区：[1]食品营养与安全教育部重点实验室天津科技大学食品工程与生物技术学院,天津300457 [2]南开大学蓖麻工程研究中心,天津300071 [3]美国农业部农科院西部研究中心,美国加利福尼亚94710

出　　处：《农产品加工（下）》2012年第6期45-49,共5页Farm Products Processing

基　　金：天津市东丽区科技创新专项资金项目(009);天津市2011年度引进国外技术;管理人才项目(Y2011013)

摘　　要：为了研究国内主要商业蓖麻品种的种质资源多样性，采用AP—PCR与RMAPD分子标记方法，对来自国内不同地区的31份蓖麻品种进行分析。AP—PCR分子标记的研究结果表明，9条引物扩增的条带为5—12条，共计82条带，平均每条引物扩增出9．1条带，扩增的多态性条带为2。10条，多态率为40％．90．91％，共计53条带，平均每条引物扩增出5．89条多态性带。在9条引物中，引物S3扩增出多态性条带最高，多态率达90．91％。蓖麻种质资源的RMAPD分析结果表明，84对引物扩增的条带为5～12条，共计85条带，平均每对引物扩增出8．5条带，扩增的多态性条带为3。10条，共计56条带，平均每条引物扩增出5．6条多态性带。在84对引物中，引物A3＋B4扩增出的条带最多，多态性最高，多态率达83．3％。对AP—PCR与RMAPD分子标记的数据进行联合聚类分析表明，在遗传系数为0．56处，31份蓖麻种质被分成3大类群。2种分子标记之间的相关系数不显著（R=0．0093）。In this paper, AP-PCR and RMAPD methods have been used to analyze 31 different kinds of Ricinus communis from different domestic regions. Results of AP-PCR show that the number of bands amplified from 9 primers ranges between 5 to 12, with an average of 9.1 bands by each primer and in a total number of 82 bands. The number of amplified polymorphic bands is between 2 to 10, with the polymorphism rate from 40% to 90.91%. 5.89 polymorphic bands on average are amplified by each primer with a total of 53 bands. Among these 9 primers, primer $3 has the highest polymorphism with the polymorphism rate 90.91%. Results of RMAPD show that the number of bands amplified by 84 pairs of primers ranges from 5 to 12, with an average of 8.5 and in total of 85; the number of polymorphic bands ranges from 3 to 10, with an average of 5.6 and in total of 56. Among 84 pairs of primers, A3＋B4 has the largest number and the highest polymorphism with the polymorphism rate 83.3%. The joint cluster analysis of AP-PCR and RMAPD suggests that 31 copies of Ricinus communis germplasm are divided into 3 categories in the section when the heritability coefficient is 0.56. The correlation coefficient of these two molecular marker methods is not significant （R=0.009 3）.

关 键 词：蓖麻 种质资源 AP-PCR RMAPD 多怼胜 

分 类 号：S565.6[农业科学—作物学]