雷帕霉素对人胃癌SGC-7901细胞增殖的影响及其分子机制  

Effect of rapamycin on human gastric cancer SGC-7901 cells and the molecular mechanism

作　　者：孔姝婧[1] 高超[2] 裴冬生[3] 赵晶[2] 朱艳卿[1] 杨欢[1] 李倩[1] 

机构地区：[1]徐州医学院研究生学院,江苏徐州221004 [2]徐州医学院附属医院肿瘤科,江苏徐州221002 [3]徐州医学院肿瘤生物治疗实验室

出　　处：《徐州医学院学报》2012年第4期219-221,共3页Acta Academiae Medicinae Xuzhou

摘　　要：目的观察雷帕霉素对人胃癌SGC-7901细胞增殖的影响及其对缺氧诱导因子1α（HIF-1α）、血管内皮生长因子（VEGF）基因表达的影响。方法SGC-7901细胞经雷帕霉素处理后，以四甲基偶氮唑蓝（MTT）法检测细胞的增殖情况，逆转录聚合酶链反应（RT—PCR）检HIF-1α、VEGF基因的表达。结果体外雷帕霉素可抑制人胃癌SGC-7901细胞增殖，并下调人胃癌SGC-7901细胞HIF—1α、VEGF基因的表达。结论雷帕霉素可抑制人胃癌SGC-7901细胞增殖，并且可能是通过下调人胃癌SGC-7901细胞HIF-1α、VEGF基因的表达而起到抑制作用的。Objective To investigate the effect of rapamycin on the proliferation of gastric cancer cell line SGC - 7901, as well as hypoxia- inducible factor-1α（ HIF-1α）, vascular endothelial growth factor （VEGF）expression. Methods Gastric cancer cells were treated with rapamycin. Cell proliferation was detected by MTT assay. Semi - quanti- tative RT - PCR was applied to detect the expression of HIF - 1α and VEGF mRNA changes. Results Rapamycin inhibited proliferation of human gastric cancer SGC -7901 cells in vitro. Rapamyein in human gastric cancer cells reduced HIF- 1α, VEGF mRNA expression. Conclusion Rapamycin can inhibit proliferation of human gastric cancer SGC -7901 cells in vitro. Rapamycin may downregulate HIF- 1α and VEGF mRNA expression in human gastric cancer cells.

关 键 词：雷帕霉素 胃癌 缺氧诱导因子1Α 血管内皮生长因子 

分 类 号：R73-362[医药卫生—肿瘤]