杏仁核去5-HT支配小鼠抑郁样行为学变化与血清ACTH和TSH浓度的关系  

The effect of 5 -HT depletion in bilateral amygdalae on the behavior of depression and serum ACTH, TSH levels in mice

作　　者：宋亮[1,3] 刘阳[1] 张继猛[1] 刘芳[2] 陈幽婷[3] 

机构地区：[1]徐州医学院神经生物学实验室,江苏徐州221002 [2]徐州医学院组织胚胎学教研室 [3]徐州医学院解剖学教研室

出　　处：《徐州医学院学报》2012年第4期234-238,共5页Acta Academiae Medicinae Xuzhou

摘　　要：目的观察小鼠双侧杏仁核去5-羟色胺（5-HT）支配后，在不同时程内所出现的抑郁样行为以及血清促肾上腺皮质激素（ACTH）和促甲状腺激素（TSH）浓度的变化，探讨杏仁核内的5-HT对下丘脑-垂体-肾上腺（HPA）轴和下丘脑-垂体-甲状腺（HPT）轴的调节以及与抑郁症的关系。方法双侧杏仁核定位注射5-HT能神经元的特异性损毁剂5，7-双羟色胺（5，7-DHT，DHT组）或生理盐水加维生素C（Vc，溶剂组），在不同时间点（术后1h、14天、21天）采血，用酶联免疫吸附法检测血清TSH和ACTH浓度。采用强迫游泳实验和悬尾实验检测小鼠抑郁样行为表现。结果与溶剂组相比，14天DHT组小鼠强迫游泳实验不动时间明显延长（P〈0．05），但21天时未见差异；14天DHT组和21天DHT组小鼠悬尾实验不动时间均明显延长（P〈0．05），21天显示差异具有极显著性（P〈0．01）；14天DHT组血清ACTH浓度显著降低（P〈0．01），至21天时较14天升高（P〈0．05），但未达到正常水平（P〈0．05）；14天DHT组血清TSH浓度显著降低（P〈0．05），至21天恢复到正常水平。结论杏仁核内5-HT浓度降低会引起小鼠抑郁样行为，并可能经HPA和HPT轴影响ACTH和TSH的分泌。经过代偿，部分激素水平可恢复，但抑郁症状并未完全消失。Objective To evaluate the effect of 5 - hydroxytryptamine （5 - HT） depletion in bilateral amygdalae by 5,7 -dihydroxytryptamine （5,7 -DHT） on the behavior of depression and adrenocorticotropic hormone （ACTH） , thy- roid stimulating hormone （TSH） level in mice, to investigate its regulation on ACTH and TSH, so as to the relationship with depression. Methods Male Kunming mice were divided into control groups and 5,7 - DHT groups; to collect ser- um samples at differrent time point （1 h, 14 d, 21 d after operation） , then to detect the ACTH and TSH level of mouse serum by enzyme linked immunosorbent assay （ELISA） ; to evaluate the depressive behavior in mice via forced swimming and tail suspension test. Results Compared with control group, the 14 day 5,7 - DHT group significantly increased the immobility time of depression model in two tests （ P 〈 0.05 ） , but only immobility time of tail suspension test increased in 21 day group. Compared with control group, the 14 day 5,7 -DHT group significantly decreased the serum ACTH and TSH levels （ P 〈 0.05 ）, and the 21 day ACTH levels were still lower than control group （ P 〈 0.05 ）, but higher than 14 day＇s, while the TSH level returned to normal. Conclusion The mice of which 5 - HT are depleted in bilateral amyg- dalae display depression behavior, while the ACTH and TSH in serum decrease significantly, but will increase to normal level gradually, while systoms of depression have not vanished absolutely.

关 键 词：5 7-双羟色胺 抑郁症 促肾上腺皮质激素 促甲状腺激素 小鼠 

分 类 号：R749.38[医药卫生—神经病学与精神病学]