高效液相色谱法测定大豆中异黄酮的含量  

Determination of Soybean Lsotlavones in HPLC

作　　者：江和源[1] 吕飞杰[1] 邰建祥 东惠茹[2] 

机构地区：[1]中国农业科学院作物育种栽培研究所,北京100081 [2]中国农业科学院蔬菜花卉研究所,北京100081

出　　处：《食品科学》2000年第4期56-58,共3页Food Science

摘　　要：用高效液相色谱法测定了大豆中六种异黄酮成分，同时比较了不同浓度乙醇制备样品对含量测定的影响。结果显示：大豆中极性异黄酮含量高，低极性异黄酮甙元含量低。测定大豆中异黄酮含量的乙醇浓度最好控制在６０％～８０％。色谱柱采用日本岛津公司的Ｓｈｉｍ－Ｐａｃｋ　ＣＬＣ　ＯＤＳ柱（ＩＳＯ×６．０ｍｍＩＤ；５μｍ），流动相为ＭｅＯＨ：ＨＡＣ：Ｈ_２Ｏ（３０：３．５：６６．５），流速为０～８．５ｍｉｎ时１．０ｍｌ／ｍｉｎ，５．８～４７ｍｉｎ时１．５ｍｌ／ｍｉｎ，柱温为５０℃，检测波长为２５４ｎｍ。Six isoflavones in the soybeans were determined by bligh performance Liquid chromatog raphy (HPLC) .The effects of the ratio of ethanol and water on the prepared sample were studied.Results showed that polar isoflavone glycosides in the soybeans were high when low-polar isoflavone agiucones were almost none.And 60%~80% aqueous ethanol was the optimal ratio in the sample prepared The samples were analysed by a Shin-Pack CLC ODS colunm (150 × 6.0mmID: 5 μ m) .The mobile phase was MeOH:HAC:H_2O(30: 3.5:66.5) .The flow rate was 1.0ml/min from 0 to 8. 5min and 1. 5ml/min from 8. 5 to 47min. Column temperature was 50 ℃, UV-detection was performed at 254um.

关 键 词：高效液相色谱 大豆 异黄酮 测定 

分 类 号：TS214.01[轻工技术与工程—粮食、油脂及植物蛋白工程] TS207.3[轻工技术与工程—食品科学与工程]