硒酸酯多糖对人食管鳞癌Eca109细胞增殖及凋亡的影响  

Effect of kappa-selenocarrageenan on proliferation and apoptosis in human esophageal squamous carcinoma Eca109 cell line

作　　者：岳黎敏[1] 杨以会[1] 赵克胜 

机构地区：[1]河北工程大学医学院,河北邯郸056029 [2]河北省邯郸矿业集团总医院麻醉科,056003

出　　处：《重庆医学》2012年第16期1612-1613,1616,共3页Chongqing medicine

摘　　要：目的研究硒酸酯多糖(KSC)对人食管鳞癌Eca109细胞增殖及凋亡的影响,并初步探讨其作用机制。方法甲基噻唑基四唑(MTT)法检测不同浓度KSC对Eca109细胞增殖的影响;流式细胞术观察KSC对Eca109细胞凋亡的影响;Westernblot技术检测KSC对Eca109细胞核转录因子-κB(NF-κB)蛋白表达水平的影响。结果 KSC可明显抑制Eca109的增殖(P<0.05),并呈时间及浓度依赖性;经KSC作用后,Eca109细胞的凋亡率随KSC作用时间的延长(24、48、72h)及KSC浓度的增加(30、60、120μg/mL)而明显升高(P<0.01);KSC(60、120μg/mL)显著下调Eca109细胞核中NF-κB蛋白水平(P<0.01)。结论 KSC对Eca109细胞具有抑制增殖、诱导凋亡作用,其作用机制可能与抑制NF-κB信号通路有关。Objective To study the effect of kappa-selenocarrageenan（KSC） on the proliferation and apoptosis of esophageal squamous carcinoma cell line Ecal09 and its mechanism. Methods After treatment with KSC at different concentrations,the inhibi- tory effect on proliferation of Ecal09 cells was measured by MTT and the apoptotic rates were analyzed with flow cytometry. Abun dance of NF-~B protein of Ecal09 cells was detected by Western-blot. Results KSC could inhibit the growth of Ecal09 cells in both dose and time dependent manners（P〈0.05）. The apoptotic rate of Ecal09 cells was significantly increased by KSC in a time-de- pendent（24,48 and 72 h） and concentration-dependent（30,60,120μg/mL） manners（P〈0.01）. Abundance of NF-κB protein was down regulated in KSC（60,120 μg/mL） treated Ecal09 cells（P〈0.01）. Conclusion KSC can inhibit the proliferation and induce apoptosis of Ecal09 cells,which is correlated with down-regulation of the abundance of NF-κB protein.

关 键 词：食管肿瘤 细胞凋亡 NF-ΚB 硒酸酯多糖 

分 类 号：R735.1[医药卫生—肿瘤]