CyclinD1和P73在口腔粘膜鳞状细胞癌化疗前后的表达及临床意义  

Expressions of CyclinD1 and P73 in buccal mucosa squamous cell carcinoma before and after chemotherapy and their clinical significance

作　　者：陈中[1] 张雨温[1] 于登臣[1] 张素欣[1] 

机构地区：[1]河北医科大学第四医院口腔科,石家庄050011

出　　处：《现代口腔医学杂志》2012年第3期145-148,179,共5页Journal of Modern Stomatology

基　　金：河北省医学科学研究重点课题(20090531)

摘　　要：目的研究CyclinD1和P73在口腔粘膜鳞状细胞癌中应用奥铂联合替加氟化疗前后的表达及临床意义。方法选取30例口腔粘膜鳞状细胞癌患者的癌组织为实验组,10例口腔粘膜良性肿瘤患者的正常黏膜为对照组。将癌组织和正常粘膜组织分为三组,即A组(化疗前)、B组(化疗后)和C组(正常粘膜)。应用免疫组织化学SP法和流式细胞术,检测CyclinD1和P73在各组中的表达。结果①免疫组化:CyclinD1在化疗前口腔粘膜鳞状细胞癌中阳性率为91.29±3.10%,化疗后为71.78±4.46%,正常黏膜组织阳性率为51.32±2.29%;P73在化疗前口腔粘膜鳞状细胞癌中阳性率为88.97±4.2%,化疗后为72.14±11.6%,正常黏膜组织阳性率为53.49±5.01%。②流式细胞术:CyclinD1在化疗前口腔粘膜鳞状细胞癌中的表达为1.89±0.10,化疗后为1.35±0.15,正常口腔粘膜细胞为1.0±0.04。各组之间比较有显著性差异(P<0.01)。P73在化疗前为1.75±0.18,化疗后为1.29±0.15,正常粘膜细胞为0.99±0.04。各组之间比较有显著性差异(P<0.01)。结论 CyclinD1和P73可能是口腔黏膜癌变的早期事件,可作为癌变监测和临床疗效观察的辅助指标。Objective To study the expressions of CyclinD1 and P73 in buccal mucosa squamous cell carcinoma before and after combination chemotherapy with Oxaliplatin（L-OHP） and Tegafur（UFT） and their clinical significance.Methods Thirty cases of cancer tissue from the patients with buccal mucosa squamous cell carcinoma were selected as experimental group;10 cases of normal mucosa from the patients with benign tumor of buccal mucosa were selected as control group.Cancer tissue and normal mucosa tissue were divided into Group A（before chemotherapy）,Group B（after chemotherapy） and Group C（normal mucosa）.The expressions of CyclinD1 and P73 in each tissue were detected by Streptavidin-Peroxidase（SP） immunohistochemical method and flow cytometry（FCM）.Results ① Immunohistochemistry： The positive expression rates of CyclinD1 in buccal mucosa squamous cell carcinoma before and after chemotherapy were 91.29±3.10% and 71.78±4.46%,respectively.The positive expression rate of CyclinD1 in normal mucosa tissue was 51.32±2.29%.The positive expression rates of P73 in buccal mucosa squamous cell carcinoma before and after chemotherapy were 88.97±4.2% and 72.14±11.6%,respectively.The positive expression rate of P73 in normal mucosa tissue was 53.49±5.01%.②FCM： The expressions of CyclinD1 in buccal mucosa squamous cell carcinoma before and after chemotherapy were 1.89±0.10 and 1.35±0.15,respectively.The expression of CyclinD1 in normal buccal mucosal cell was 1.0±0.04.There were significant differences among the groups（P0.01））.The expressions of P73 before and after chemotherapy were 1.75±0.18 and 1.29±0.15,respectively.The expression of P73 in normal mucosal cell was 0.99±0.04.There were significant differences among the groups（P0.01）.Conclusion CyclinD1 and P73 may be the early events of carcinogenesis of buccal mucosa,which can be used as the auxiliary indicators for cancer monitoring and observation of clinical efficacy.

关 键 词：CYCLIND1 P73 口腔粘膜 鳞状细胞癌 免疫组织化学 流式细胞术 

分 类 号：R739.8[医药卫生—肿瘤]