流动注射-吸光光度法测定葡萄糖共存下的果糖  

DETERMINATION OF FRUCTOSE IN THE PRESENCE OF GLUCOSE BY FLOW INJECTION-ABSORPTIOPHOTOMETRY

作　　者：许汉英[1] 赵晓君[2] 

机构地区：[1]吉林进出口商品检验局,长春130062 [2]吉林大学化学系

出　　处：《理化检验（化学分册）》2000年第3期102-103,105,共3页Physical Testing and Chemical Analysis(Part B:Chemical Analysis)

摘　　要：利用低温下葡萄糖和果糖与 K3[Fe( CN) 6]反应速率的明显差异 ,采用流动注射 -吸光光度法测定了葡萄糖存在下的果糖。该法对果糖的测定范围为 2 .0～ 2 0 mg· ml- 1 ,当 C果 /C糖 ≥ 1 .0时 ,葡萄糖无明显干扰。对蜂蜜样品进行测定并与滴定法对照 ,相对误差为 - 2 .8%～ 2 .9%。Based on the significant difference of reaction rates between glucose and fructose reacting with K 3[Fe(CN) 6] at low temperature, a novel flow injection absorptiophotometric method for determination of fructose in the present of glucose is proposed in this paper. Fructose can be determined in the linear range of 2.0~20mg·ml -1 . At the ratio of C fructose / C glucose ≥1.0, there is no significant interference from glucose. The proposed method has been used for determination of fructose in honey and as compared with the titration method, the relative error is between -2.8%~2.9%.

关 键 词：FIA-SP 果糖 葡萄糖 蜂蜜 光度法 

分 类 号：S896.1[农业科学—特种经济动物饲养] O657.32[农业科学—畜牧兽医]