基于药效理化表征的当归补血汤质量控制模式初探  

Preliminary study on quality control model of Danggui Buxue Decoction based on physicochemical characterization

作　　者：马家骅[1] 李霞[2] 谭承佳[3] 贺福元[4] 李楠[2] 杨明[2] 

机构地区：[1]西南科技大学生物科学实验中心,四川绵阳621010 [2]成都中医药大学,四川成都611137 [3]绵阳师范学院,四川绵阳621000 [4]湖南中医药大学,湖南长沙410004

出　　处：《中草药》2012年第5期901-905,共5页Chinese Traditional and Herbal Drugs

基　　金：国家自然科学基金资助项目(30801550);西南科技大学博士基金资助项目(10zx7153)

摘　　要：目的研究当归补血汤不同体系与其理化参数的关系,探讨利用药效理化参数来表征中药汤剂质量的可行性。方法以水为分散介质,参照不同分散体系的研究方法,对黄芪甲苷、阿魏酸、黄芪皂苷、黄芪多糖等当归补血汤的代表性组分及当归补血汤单味药、复方提取液的理化特征进行表征,建立包括表面特性、电性质、流变性、热力学等性质的表征方法,并进行参数与处理因素的多项式回归分析。结果随着阿魏酸质量浓度增大,阿魏酸水溶液、当归水提液、复方水提液的pH值降低、电导率增大;随着黄芪甲苷与黄芪皂苷质量浓度的增大,黄芪甲苷水溶液、黄芪皂苷水溶液、黄芪水提液、复方水提液的表面张力降低;随着多糖质量浓度的增大,复方水提液的渗透压增大。在整个当归补血汤体系中影响pH值和电导率的主导成分是阿魏酸,影响表面张力的主导组分是黄芪皂苷,影响渗透压的主导组分为多糖,且不同工艺制备的汤剂的理化参数大小趋势与药效强弱也有较强的相关性。结论初步表明表面张力、pH值、电导率、渗透压4个参数能反映当归补血汤的内在质量,在一定程度上可以作为当归补血汤制备工艺与汤剂质量控制的参考指标。Objective To study the relationships between different disperse systems and physicochemical parameters of Danggui Buxue Decoction（DBD）,and to explore the feasibility of physicochemical characterization in pharmacology on the quality of DBD.Methods Solutions of ferulic acid,astragaloside IV,astragaloside,astragalus polysaccharides,single herb,and DBD with different concentration or technology were made,of which physicochemical parameters at 25 ℃ were determined such as surface tension,pH value,conductivity,and osmotic pressure,etc.And polynomial regression analysis was used to analyze these data.Results With the increase of ferulic acid concentration,pH value was decreased while conductivity was increased in the aqueos solutions of ferulic acid,Angelicae Sinensis Radix,and DBD.And the surface tension was decreased in the aqueous solution of astragaloside IV,astragaloside,Astragali Radix,and DBD.In the combined system of DBD,ferulic acid was the main factor influencing pH value and conductivity,while astragaloside was the main factor influencing surface tension,and polysaccharide was the main factor influencing osmotic pressure.Furthermore,there was comparatively intimate correlation between physicochemical parameters and pharmacodynamic action.Conclusion These results preliminarily suggest that characteristic parameters,such as surface tension,pH value,conductivity,and osmotic pressure,could be used to characterize the decoction quality and provide reference for preparation

关 键 词：当归补血汤 质量控制 黄芪甲苷 阿魏酸 理化表征 黄芪皂苷 黄芪多糖 

分 类 号：R283.6[医药卫生—中药学]