烫伤合剂中没食子酸和绿原酸的测定  

Determination of Gallic Acid and Chlorogenic Acid in Tangshang Mixture by HPLC

作　　者：王亚洲[1] 

机构地区：[1]广西医科大学第四附属医院,广西柳州545005

出　　处：《中国民族民间医药》2012年第9期45-46,共2页Chinese Journal of Ethnomedicine and Ethnopharmacy

摘　　要：目的:建立烫伤合剂中没食子酸和绿原酸的测定方法。方法:采用反相高效液相色谱法法进行定量分析,用Inertsil C8-3柱,乙腈-0.1%磷酸-四氢呋喃(14∶85∶1)为流动相,流速为1.0 mL.min-1,检测波长为290 nm。结果:没食子酸和绿原酸的线性范围分别为21.55～215.5μg.mL-1和25.45～254.5μg.mL-1;平均加样回收率分别为98.40%和99.99%(n=6)。结论:此法简便、准确,具有良好的重现性和回收率,适用于烫伤合剂中没食子酸和绿原酸的同时测定。Objective： To establish a method for determination of the content of gallic acid and chlorogenic acid in tangshang mixture. Method： The quantitative analysis was carried out by HPLC with a Inertsil C8 -3 column. The mobile phase was acetonitrile - 0. 1% phosphoric acid- tetrahydrofuran （14： 85：1 ） with a flow rate at 1.0 mL . min-1. Detection wavelength was 29Ohm. Resuits： The linear ranges for gallic acid and chlorogenie acid were 21.55 ~ 215.5 μg . mL- 1 and 25.45 -254. 5 μg .mL- 1 respectively. The average recoveries were 98. 40% and 99. 99% （n = 6） . Conclusion： This method is simple and accurate, with good repeatability and recovery. It is suitable for the determination of the content of gallic acid and chlorogenic acid in tangshang mixture.

关 键 词：高效液相色谱法 烫伤合剂 没食子酸 绿原酸 

分 类 号：R284.1[医药卫生—中药学]