MALP-2对多形核中性细胞凋亡及其Bax蛋白表达的影响  

Effects of MALP-2 on apoptosis and intracellular Bax expression of neutrophils

作　　者：高燕[1] 叶迎春[2] 袁青[1] 周云刚[1] 黄黎[1] 刘佳佳[1] 

机构地区：[1]泸州医学院免疫学教研室,四川泸州646000 [2]泸州医学院病原生物与免疫学实验室,四川泸州646000

出　　处：《重庆医学》2012年第14期1353-1354,1357,共3页Chongqing medicine

基　　金：国家自然科学基金资助项目(30371305)

摘　　要：目的研究Toll样受体(TLR)激动剂巨噬细胞活化脂肽(MALP-2)影响多形核中性细胞(PMN)凋亡及凋亡相关的Bax蛋白的表达水平。方法分离培养正常受试者的外周血PMN,分别加入实验组、阳性对照组和阴性对照组。用流式细胞术检测不同组PMN的凋亡发生率,通过Western blotting技术检测各组PMN Bax蛋白的表达水平。结果 MALP-2降低PMN自发性凋亡的发生率,与阳性对照组和阴性对照组比较差异有统计学意义(P<0.05);接受MALP-2刺激的PMN表达Bax蛋白低于阳性对照组与阴性对照组(P<0.05)。结论在TLR激动剂诱发的PMN生存效应中,MALP-2可能通过降低PMN胞内Bax蛋白的表达水平来延长PMN的生命期限。Objective To explore the effects of MALP-2 as TLR agonist on the apoptosis and intracellular Bax protein expression of polymorphonuclear neutrophils（PMN）.Methods Heparinized peripheral blood was obtained from healthy volunteers.PMN were cultured according to experimental needs：the experimental group（MALP-2）,positive control group（flagellin） and negative control group.Apoptosis of PMN was detected by flow cytometric analysis.The expression of Bax protein of PMN was examined by Western blotting analysis.Results Flow cytometry indicated that MALP-2 delayed PMN apoptosis.Western blotting showed that the expression of Bax protein in PMN was down-regulated by MALP-2.Conclusion MALP-2 might delay spontaneous apoptosis of PMN by reducing the expression of Bax protein in PMN.

关 键 词：中性核细胞 细胞凋亡 TOLL样受体 BCL-2相关X蛋白质 

分 类 号：R363[医药卫生—病理学]