双孔钾通道TREK-1在神经细胞正常及缺氧条件下的表达研究  

Expression of TREK-1 on Cultured Neuron and Astrocytes under Normal and Hypoxia Condition

作　　者：吴潇[1] 谢敏杰[1] 唐荣华[1] 

机构地区：[1]华中科技大学同济医学院附属同济医院神经内科,武汉430030

出　　处：《神经损伤与功能重建》2012年第3期166-169,共4页Neural Injury and Functional Reconstruction

基　　金：国家自然科学基金(No.30971007)

摘　　要：目的:研究双孔钾通道TREK-1在神经元及星形胶质细胞的表达,及在缺氧时星形胶质细胞中的表达变化。方法:离体培养大鼠皮质神经元和星形胶质细胞,利用免疫荧光染色法观察TREK-1蛋白在神经元和星形胶质细胞的表达分布,对星形胶质细胞进行缺氧干预,实时PCR观察缺氧不同时间TREK-1的表达变化。结果:TREK-1通道在神经元和星形胶质细胞均有表达,缺氧时TREK-1在星形胶质细胞中呈先上升,后逐渐下降的趋势。结论:TREK-1在神经元和星形胶质细胞中均有表达,星形胶质细胞中TREK-1的表达在缺氧损伤中发生动态变化。Objective：To investigate the expression of two-pore domain potassium channel—TREK-1 on primary cultured neurons and astrocytes and the temporal changes of TREK-1 expression in astrocytes under hypoxia insult.Methods： The cortical neurons and astrocytes were cultured.The expression of TREK-1 in cultured neurons and astrocytes were analyzed by double immunofluorescence staining.The temporal changes of TREK-1 expression in astrocytes under hypoxia insults were detected by Real-time PCR.Results： The TREK-1 immuno-reactivity was expressed on both the neurons and astrocytes under normal condition.During hypoxia insult,the TREK-1 expression on astrocyte increases at early phases and decreases gradually at late phase.Conclusion： The TREK-1 immunoreactivity is expressed on both the neurons and astrocytes.In astrocytes under hypoxia,there are temporal changes on TREK-1 expression.

关 键 词：TREK-1 神经元 星形胶质细胞 缺氧 

分 类 号：R741[医药卫生—神经病学与精神病学] R741.02[医药卫生—临床医学]