胰岛素样生长因子结合蛋白5对人骨肉瘤细胞增殖与侵袭的影响  

Effects of Insulin-like Growth Factor Binding Protein 5 on the Proliferation and Invasion of Osteosarcoma Cells

作　　者：周杰[1,2] 覃佳强[1,2] 金先庆[2] 宿玉玺[1,2] 谢艳[3] 南国新[1,2] 王忠良[1,2] 刘星[1,2] 张德文[1] 

机构地区：[1]重庆医科大学附属儿童医院骨科 [2]儿童发育疾病研究省部共建教育部重点实验室,重庆市400014 [3]重庆医科大学第一附属医院检验科

出　　处：《中国肿瘤临床》2012年第10期626-629,共4页Chinese Journal of Clinical Oncology

基　　金：国家自然科学基金(编号:81001197);重庆市自然科学基金(编号:CSTC;2010BB5109)资助~~

摘　　要：目的:探讨胰岛素样生长因子结合蛋白5(insulin-like growth factor binding protein 5,IGFBP5)对人骨肉瘤细胞株143B增殖及侵袭力的影响。方法:用IGFBP5过表达重组腺病毒(Ad-IGFBP5)、IGFBP5 RNA干扰重组腺病毒(Ad-siIGFBP5)和空载红色荧光蛋白重组腺病毒(Ad-RFP)感染人骨肉瘤细胞株143B。通过细胞增殖、划痕愈合、细胞迁移室(Transwell)侵袭等多种实验方法,研究IGFBP5对人骨肉瘤细胞株生长、迁移与侵袭的影响。结果:Ad-IGFBP5组感染后143B细胞生长速度低于Ad-RFP组(P<0.05);Ad-IGFBP5组细胞在划痕后16h即完全愈合,划痕愈合速度显著低于Ad-RFP组(P<0.05);24 h后Ad-IGFBP5组侵袭至Matrigel胶上的平均细胞数量较Ad-RFP组减少(P<0.05)。而Ad-siIGFBP5感染后,143B细胞表现为生长率升高,划痕愈合速度加快及侵袭力增强。结论:IGFBP5能抑制骨肉瘤细胞增殖、迁移、侵袭。提示IGFBP5在抑制骨肉瘤发生及转移中可能起到重要作用。Objectives： This work aims to investigate the effect of insulin-like growth factor binding protein 5 （ 1GFBP5 ） on the proliferation and invasion of human osteosarcoma cells （ 143B ）. Methods： The effects of IGFBP5 on the growth, migration, and invasion of osteosarcoma 143B cells were studied. The 143B cell lines were infected with IGFBP5-overexpressing recombinant adenovirus （ Ad - IGFBP5 ） and small interfering RNA-mediated knockdown of IGFBP5 recombinant adenovirus （ Ad - siIGFBP5 ）. Cell proliferation, cell wound healing assay, and Transwell invasion assay were performed to determine the effect of IGFBP5 on the osteosarcoma cells. Results： Cell proliferation assay and fimctional analysis revealed that the osteosarcoma cells infected with Ad-IGFBP5 had significantly lower cell growth rates, wound closure rates, and invasiveness compared with the cells in the control group （ P 〈 0.05 ）. Meanwhile, after infection with Ad-siIGFBP5, the cells exhibited the opposite biological effects. Conclusion： 1GFBP5 may inhibit the tumorigenesis and metastasis ofosteosarcoma.

关 键 词：胰岛素样生长因子结合蛋白5 重组腺病毒 骨肉瘤 肿瘤抑制 

分 类 号：R738.1[医药卫生—肿瘤]