自噬在吉西他滨诱导肺癌细胞A549凋亡中的作用及其相关机制  

Effect of Autophagy on Gemcitabine-induced A549 Lung Cancer Cell Death and Relevant Mechanisms

作　　者：朱良纲[1] 杭均彪[1] 杜海磊[1] 陈聆[1] 施敏敏[2] 陈凯[1] 项捷[1] 周翔[1] 杨孝清[1] 车嘉铭[1] 任健[1] 陈中元[1] 

机构地区：[1]上海交通大学附属瑞金医院胸外科,上海市200025 [2]上海交通大学附属瑞金医院消化外科研究所

出　　处：《中国肿瘤临床》2012年第10期652-655,共4页Chinese Journal of Clinical Oncology

基　　金：上海优秀青年教师科研专项基金(编号:jdy10149)资助~~

摘　　要：目的:研究自噬对吉西他滨(gemcitabine,GEM)诱导的肺癌细胞A549凋亡的影响并探讨其可能机制。方法:MDC染色后采用荧光显微镜对自噬进行形态观察;以CCK8检测3-MA抑制自噬前后经吉西他滨诱导的A549细胞存活率;RT-PCR检测自噬相关基因Beclin-1表达的变化。Western blot分别检测自噬相关性蛋白Beclin-l及凋亡活化蛋白Caspase-3的变化。结果:吉西他滨可诱导肺癌细胞A549产生自噬,自噬在基因及蛋白水平表达均增加;3-MA和吉西他滨联合用药可增强肺癌A549细胞凋亡。结论:吉西他滨诱导肺癌细胞A549凋亡的过程中自噬可能起到保护作用,3-MA特异性抑制自噬后可增强吉西他滨诱导的A549细胞凋亡。Objectives： To investigate the mechanism and effects of autophagy on gemcitabine-induced A549 lung cancer cell death. Methods： Quantitative analysis of autophagy after monodansylcadaverine （ MDC ） staining was performed using fluorescence microscopy. The cell viability of A549 cells after gemcitabine treatment in the presence or absence of autophagy inhibition was determined using eholecystokinin octapeptide （ CCK8 ）. Real-time polymerase chain reaction （RT-PCR） was used to measure the expression of the au- tophagy-related gene Beclin-1. Western blot analysis was used to determine the Beclin-1 and caspase-3 protein levels. Results： Autophagy is involved in the gemcitabine-induced A549 lung cancer cell death. The levels of both autophagy-related genes and proteins are increased. In addition, inhibition of autophagy promoted apoptosis in the A549 lung cancer cells. Conclusion： Autophagy protects A549 lung cancer cells against gemcitabine-induced apoptosis. Moreover, cell apoptosis can be enhanced by the inhibition of autophagy.

关 键 词：非小细胞肺癌 自噬 凋亡 吉西他滨 CASPASE-3 

分 类 号：R734.2[医药卫生—肿瘤]