乙酰肝素酶、基质金属蛋白酶2和金属蛋白酶组织抑制剂2在恶性黑素瘤的表达  

Expressions of heparinase, matrix metalloproteinase 2 and tissue inhibitor of metalloproteinase 2 in malignant melanoma

作　　者：陈建立[1] 张江安[1] 于建斌[1] 苏蓓蓓[1] 孟静[1] 

机构地区：[1]郑州大学第一附属医院皮肤科,450052

出　　处：《中华皮肤科杂志》2012年第6期422-425,共4页Chinese Journal of Dermatology

摘　　要：目的 探讨乙酰肝素酶、基质金属蛋白酶2（MMP2）和金属蛋白酶组织抑制剂2（TIMP2）蛋白在恶性黑素瘤皮损中的表达及意义。方法 选择恶性黑素瘤30例、黑素细胞痣30例、正常皮肤组织15例，分别采用免疫组化SP法检测皮损中乙酰肝素酶、MMP2和TIMP2蛋白的表达。结果 恶性黑素瘤皮损乙酰肝素酶蛋白的阳性表达率与黑素细胞痣、与正常皮肤组织相比，差异均有统计学意义（χ21 = 21.172，P 〈 0.01；χ22 = 27.805，P 〈 0.01）。恶性黑素瘤皮损MMP2蛋白的阳性表达率与黑素细胞痣、与正常皮肤组织相比，差异均有统计学意义（χ21 = 19.817，P 〈 0.01；χ22 = 19.866，P 〈 0.01）。恶性黑素瘤皮损TIMP2蛋白的阳性表达率与黑素细胞痣、与正常皮肤组织相比，差异均有统计学意义（χ21 = 19.200，P 〈 0.01；χ22 = 15.000，P 〈 0.01）。结论 乙酰肝素酶、MMP2和TIMP2蛋白在恶性黑素瘤中的表达水平明显高于其在黑素细胞痣和正常皮肤中的表达水平。Objective To investigate the expressions of heparinase, matrix metalloproteinase 2 （MMP2） and tissue inhibitor of metalloproteinase 2 （TIMP2） in malignant melanoma lesions and their significance. Methods Skin specimens were obtained from the lesions of 30 patients with malignant melanoma, 30 patients with melanocytic nevus and the normal skin of 15 healthy controls. Immunohistochemical SP method was used to detect the protein expression of heparinase, MMP2 and TIMP2. Results The malignant melanoma tissue specimens significantly differed from the melanocytic nevus and control tissue specimens in the expression rate of heparinase （63.33% vs. 6.67% and 0.00, χ2 = 21.172, 27.805, both P 〈 0.01）, MMP2（70.00% vs. 13.33% and 0.00, χ2 = 19.817, 19.866, both P 〈 0.01） and TIMP2 （60.00% vs. 6.67% and 0.00, χ2 = 19.200, 15.000, both P 〈 0.01）. Conclusion The expression of heparinase, MMP2 and TIMP2 is significantly higher in malignant melanoma lesions than in melanocytic nevus lesions and normal skin tissue.

关 键 词：恶性黑素瘤 乙酰肝素酶 MMP2 TIMP2 

分 类 号：R739.5[医药卫生—肿瘤]