自由活动大鼠丘脑底核单细胞放电慢性在体记录的微电极阵列设计  

A microelectrode array for chronic in vivo single-unit recording in subthalamic nucleus of freely behaving rats

作　　者：郑小斌[1] 柯以铨[1] 张世忠[1] 姜晓丹[1] 张旺明[1] 徐江平[2] 程玉芳[2] 袁欣[2] 

机构地区：[1]南方医科大学珠江医院神经外科,广东神经外科研究所,广东省脑功能修复与再生重点实验室,广州510280 [2]南方医科大学神经药理学实验室,广州510515

出　　处：《中华神经医学杂志》2012年第6期594-599,共6页Chinese Journal of Neuromedicine

基　　金：国家自然科学基金（81172416/H1618）;广东省科学计划项目（20098030801375）;国家临床重点专科建设项目

摘　　要：目的探讨清醒状态下丘脑底核（STN）长期在体多通道单细胞放电记录新技术。方法采用自行设计的集束式微电极阵列记录STN单细胞放电情况。设计主要由3部分构成：（1）29号皮下注射用不锈钢管为引导管固定电极丝，并作为脑组织接地电极；（2）5根20μm铂铱合金丝作为记录电极，1根50．8μm不锈钢合金丝作为局部参考电极，电极尖端呈“路标手”样排列，聚乙二醇固定；（3）带有螺丝旋紧的连接器减少实验动物自由活动带来的信号伪迹。将这种电极用于6只正常清醒大鼠STN单细胞放电记录，评价术后5周内的STN单细胞放电单位的记录稳定性。结果术中记录到27个STN放电单位，70．4％（19／27）存活超过2d：5周内记录到9个新生放电单位。术后峰峰波幅与时间之间无显著线性相关性（r=-0．047，P=0．655）；并且信噪比维持稳定，其与峰峰波幅间存在显著线性相关（r=0．934，P=0．000）。电极植入3周，仍有超过50％的细胞保留率。相同神经元75％波形相似系数大于0．90。结论本研究设计的微电极阵列在记录活动状态下STN单细胞放电时．波形特征稳定．细胞保留率理想，适用于慢性清醒状态在体STN多通道单细胞放电的电生理记录．Objective To introduce a bundled microelectrode array of chronic in vivo single-unit recording in subthalamic nucleus （STN） of freely behaving rats. Methods STN single cell discharge was recorded by a self-designed bundled microelectrode array. Our microelectrode array design consisted of 3 parts： （1） a 29-guage stainless steel tube was served as a guide tube to facilitate the brain penetration ofmicrowire tips, and as a ground electrode of the brain tissues as well. （2） Five 20μm platiniridium wires were used as recording electrodes and a 50.8 μm stainless steel wire with 1 mm bare tip was employed as local potential reference; all the above resembled a guiding-hand-shaped arrangement in microwire tips, and was fixed by tiny plot of carbowax moreover. （3） A male connector with end screw receptors was employed to make the array connection more stable so as to minimize the movement artifacts; six normal rats were implanted with this kind of electrode arrays, and the stability of STN single-unit recording was evaluated in the following 5 weeks. Results Twenty-seven firing units were captured during operation, of which 70.4% （19/27） survived more than 2 d, and 9 new units were acquired within 5 weeks. No significant linear correlation of peak-to-peak amplitude was noted between each 2 different recording sections （Pearson r=-0.047, P=-0.655）, and whilst signal-to-noise ratio was stable with significant correlation to peak-to-peak amplitude （r=0.934, P=0.000）. More than half of the initial acquired STN units retained more than 3 weeks. And there were more than 75% r values of waveform similarities large than 0.90 of the same units across different periods. Conclusion This methodology may be appreciated for STN long-term single-unit recording with stable recording quality and favorable cell retained rate.

关 键 词：丘脑底核 微电极阵列 单细胞记录 自由活动 

分 类 号：R742.5[医药卫生—神经病学与精神病学]