VIL-10下调鼻咽癌细胞TAP-1基因表达的研究  

Down-regulation of the expression of TAP-1 by VIL-10

作　　者：任艳鑫[1] 李晓江[1] 喻博[1] 马静[2] 赵留芳[1] 杨洁[1] 隋军[1] 孙强民[3] 龙海庭[3] 

机构地区：[1]昆明医学院第三附属医院头颈肿瘤研究中心,云南昆明650118 [2]昆明市儿童医院耳鼻咽喉科,云南昆明650034 [3]中国医学科学院医学生物学研究所,云南昆明650118

出　　处：《中国耳鼻咽喉头颈外科》2012年第5期255-257,共3页Chinese Archives of Otolaryngology-Head and Neck Surgery

基　　金：云南省社会发展科技计划(应用基础研究项目)(2009ZC119M);2011年云南省卫生科技内设研究机构项目资助(2011WS0068);云南省社会发展科技计划(应用基础研究重点项目)(2009CC026)联合资助

摘　　要：目的研究VIL-10对鼻咽癌细胞株TAP-1表达的影响。方法收集在不同时间不同浓度VIL-10处理的鼻咽癌细胞,RT-PCR检测TAP-1的表达。结果①10 ng/mlVIL-10处理的CNE-1、CNE-2和N-2-BMI-1细胞株在24、48 h时TAP-1表达无明显变化;②100 ng/ml VIL-10处理的CNE-1、CNE-2和N-2-BMI-1细胞株在24 h时TAP-1表达明显减弱。结论 100 ng/ml VIL-10在24 h可明显抑制TAP-1表达,EB病毒在鼻咽癌免疫抑制过程中起一定作用。OBJECTIVE To investigate the influence of VIL-10 on the expression of TAP-1 in NPC cell lines.METHODS The NPC cells were treated by different concentration of VIL-10,and then harvested on different time.The expression of TAP-1 was detected by RT-PCR.RESULTS The cell lines of CNE-1,CNE-2 and N-2-BMI-1 were treated by VIL-10 with the concentration 10ng/ml,and the expression of TAP-1 in the 3 cell lines were not changed significantly at 24 hours and 48 hours.When the 3 cell lines were treated by VIL-10 with the concentration 100 ng/ml,the expression of TAP-1 at 24 hours were significantly decreased,but that at 48 hours were not significantly decreased.CONCLUTION The expression of TAP-1 were inhibited by VIL-10 with the concentration 100 ng/ml at 24 hours in NPC cell lines.The results indicate that EB Virus could induce immunosuppression in NPC patients.

关 键 词：鼻咽肿瘤 载体蛋白质类 逆转录聚合酶链反应 疱疹病毒4型 人 病毒白介素-10 

分 类 号：R739.63[医药卫生—肿瘤]