17-β雌二醇对牙龈卟啉单胞菌作用下人牙周膜细胞表达白细胞介素6、8的影响  

17-β estradiol promotes the expression of interleukin-6 in human periodontal ligament cells infected with Porphyromonas gingivalis

作　　者：唐晓琳[1] 刘静波[1] 包穆蓉[1] 潘亚[1] 萍钟鸣[2] 

机构地区：[1]中国医科大学口腔医学院牙周科,沈阳110002 [2]中国医科大学口腔医学院中心实验室,沈阳110002

出　　处：《中华口腔医学杂志》2012年第6期329-334,共6页Chinese Journal of Stomatology

基　　金：辽宁省博士科研启动基金（20091117）

摘　　要：目的观察17-β雌二醇（estradiol，E2）对牙龈卟啉单胞菌（Porphyromonas gingivalis，Pg）W83作用下人牙周膜细胞（human periodontal ligament cells，hPDLC）白细胞介素（interleukin，IL）6和IL-8表达的影响。方法原代培养hPDLC，传至第4代，分别以①10^-30 mol／LE2（E：1组）；②10^-7mol／LE2（E22组）；③感染复数为10：1的Pg（Pgl组）；④感染复数为100：1的Pg（Pg2组）；⑤感染复数为100：1的魄＋10^-10mol／LE2（Pg2＋E21组）；⑥感染复数为100：1的魄＋10^-7 mol／LE，（Pg2＋E，2组）处理hPDLC12和24h；以0．1％无水乙醇为对照组，酶联免疫吸附测定法检测细胞IL-6和IL-8蛋白的表达水平，实时荧光定量反转录聚合酶链法检测24hmRNA水平。采用单因素方差分析比较各组IL-6、IL-8蛋白和mRNA表达水平的组间差异，最小显著性差异事后比较检验进行组间差异的两两比较，采用独立样本t检验分析各组12和24hIL-6、IL-8表达水平的差异，检验水准为双侧d=0．05。结果Pg2组24h时IL-6蛋白表达水平[（2482．88±26．53）ng／L]显著高于Pgl组[（734．09±87．90）ng／L]、对照组[（425．8±77．25）ng／L]和12h时的Pg2组[（1157．50±234．65）ng／L，P=0．000]；pg2组24h时IL-8蛋白表达水平[（4965．81±1072．55）ng／L]显著高于Pgl组[（803．51±162．08）ng／L，P=0．007]、对照组[（400．75±2．27）ng／L，P=0．005]和12h时的Pg2组[（1431．12±82．78）ng／L，P=0．001]。E2对hPDLCIL-6和IL-8的表达无显著调节作用。与感染复数100：1的Pg单独作用相比，E：和Pg联合处理24h显著促进了hPDLCIL-6的表达水平，而未影响IL-8的表达水平：Pg2＋E：1组、Pg2＋E：2组IL-6mRNA相对于磷酸甘油醛脱氢酶的表达水平（分别为0．49±0．15、0．53±0．16）显著高于Pg2组（0．19±0．06）（P=0．021，P=0．036），两组IL_6蛋白水平[分别为（5512．66±1022．07）、（6988．78±2279．13）ng／L]�Objective To investigate the effects of 17-13 estradiol （E2 ） and Porphyromonas gingivalis（Pg）W83 on the expression of interleukin（ IL）-6 and IL-8 in human periodontal ligament cells （hPDLC）. Methods Primary cultures of hPDLC were established and the cells of passage four were treated with 10-^10 mol/L E2, 10^-7 mol/L E2 or PgW83 individually or E2 combined with PgW83. The expression levels of IL-6 and IL-8 protein at 12 h and 24 h were measured with enzyme-linked immunosorbent assay and the levels of mRNA at 24 h were detected with real-time reverse transcriptase polymerase chain reaction. Results The expression level of IL-6 reached （ 2482. 88 ± 26. 53 ） ng/L in hPDLC treated with Pg at multiplicity of infection（MOI） of 100 for 24 h, which was significantly higher than that in hPDLC treated withPgat MOI of 10：1 [（734.09±87.90） ng/L, P=0.000], the controls [（425.8 ±77.25） ng/L, P = 0. 0001 and that in hPDLC treated with Pg at MOI of 100 for 12 h [ （ 1157.50 ± 234. 65 ） ng/L, P = 0. 000]. The expression level of IL-8 reached （4965.81 ± 1072. 55 ） ng/L in hPDLC treated with Pg at MOI of 100 for 24 h, which was significantly higher than that in hPDLC treated with Pg at MOI of 10 [ （803.51 ± 162. 08） ng/L, P =0. 007], the controls [ （400. 75 ±2. 27） ng/L, P =0. 005] and that in hPDLC treated with Pg at MOI of 100 for 12 h [ （ 1431.12 ± 82. 78） ng/L, P = 0. 0011- E2 did not show remarkable effect on the expressions of IL-6 and IL-8. E2 combined with Pg （MOI = 100：1 ） significantly promoted the expression levels of IL-6 at 24 h while did not influence those of IL-8. The relative mRNA level of IL-6 in hPDLC treated with 10^-10 mol/L E2 or 10 -7 mol/L E2 combined with Pg were 0.49 ± 0. 15 （ P = 0. 021 ） and 0. 53 ±0. 16（P = 0. 036） individually, which were significantly higher than that treated with Pg alone, 0. 19 ±0.06. The protein level of IL-6 in hPDLC treated with 10^-l0 moL/L E2 or 10-7 mol/L E2 combined with Pgwere （5512.66±1022.07�

关 键 词：雌二醇 紫单胞菌 龈 人牙周膜细胞 炎症因子 

分 类 号：R781.4[医药卫生—口腔医学]