人慢性根尖周炎中白细胞介素17F的定位表达研究  

Expression of interleukin-17F in human apical periodontitis lesions

作　　者：孙辉[1] 李颂[1] 

机构地区：[1]安徽医科大学口腔医学院牙体牙髓科,合肥230032

出　　处：《中华口腔医学杂志》2012年第6期345-349,共5页Chinese Journal of Stomatology

摘　　要：目的初步探讨白细胞介素（interleukin，IL）17F在人根尖周炎病损组织中的表达情况，以期为根尖周炎的发病机制研究提供依据。方法收集安徽医科大学口腔医学院口腔颌面外科2009年11月至2011年8月经根尖手术切除的根尖周炎病损组织并做病理鉴定。经过组织切片、HE染色和显微镜病理观察，共有根尖囊肿33例，根尖肉芽肿18例。将这些组织作为试验组用于免疫组织化学分析IL-17F的表达情况，用磷酸盐缓冲液代替-抗作阴性对照，根尖囊肿和根尖肉芽肿阴性对照组各5例。收集埋伏阻生智齿拔除时需凿骨患者的健康牙槽骨质5例，作为正常对照组。对根尖囊肿和根尖肉芽肿中IL一17F的蛋白平均吸光度值（A值）进行方差齐性检验，若方差齐则行￡检验，若方差不齐则行秩和检验，检验水准为α=0．05。结果IL-17F在两组慢性根尖周炎组织中的上皮细胞、淋巴样细胞、成纤维细胞和内皮细胞中均有表达；正常对照组和阴性对照组未见IL-17F的表达。根尖囊肿中IL-17F的蛋白平均积分吸光度值（A值）为0．8852±0．0576，根尖肉芽肿为0．2365±0．0708，二者差异有统计学意义（Z=3．735，P〈0．01）。结论IL-17F存在于人根尖周炎病损组织中；IL-17F可能参与了人根尖周炎疾病的发病进程，且IL-17F在根尖囊肿和根尖肉芽肿中的表达强度具有差异性。Objective To examine the expression of interleukin-17F （IL-17F） in apical periodontitis lesions. Methods Human apical periodontitis lesions were collected after periapical surgery from November 1,2009 to August 1,2011. After histological processing, serial sectioning, HE staining, and microscopic analysis, 33 periapieal cysts and 18 periapical granulomas were selected for immunohistochemical analysis for IL-17F. Five alveolar bone samples were obtained from a group of impacted third molars recommended for extraction. These bone samples did not show any inflammation and were used as normal samples. Differences in IL-17F expression between the tissues with periapical cyst and periapica granuloma were subsequently analyzed by rank-sum test. Results IL-17F was expressed in epithelial cells,lymphocytes, fibroblast and endothelial cells of both types of lesions. Both types of lesions presented increased IL-17F expression when compared to healthy periapical alveolar bone. The expression of IL-17F in apical cyst （ 0. 2365 ± 0. 0708 ） and apical granuloma （ 0. 8852 ± 0. 0576 ） was significantly different（Z = 3. 735 ,P 〈 0. 01 ）. Condusions IL-17F could be observed and might be involved in the inflammatory response and bone resorption of periapical tissues. The expression of IL-17F in periapical cyst and periapical granuloma was significantly different.

关 键 词：根尖周炎 白细胞介素17F 根尖囊肿 根尖肉芽肿 

分 类 号：R781.34[医药卫生—口腔医学]