CD4^+与T淋巴细胞干扰素γ/诱导型一氧化氮合酶/一氧化氮通路在脐带间充质干细胞移植治疗重症肌无力中的作用  

Role of CD4^+ and interferon-γ-inducible nitric oxide synthane-nitric oxide pathway in treatment of myasthenia gravis by transplantation of umbilical cord mesenchymal stem cells

作　　者：刘红艳[1,2,3] 郭静明 王海燕[1,2,3] 叶松 冉昌丽[1,2,3] 

机构地区：[1]三峡大学第一临床医学院 [2]宜昌市中心人民医院血液科 [3]三峡大学肿瘤研究所,湖北省宜昌市443003

出　　处：《中国组织工程研究》2012年第19期3467-3470,共4页Chinese Journal of Tissue Engineering Research

摘　　要：背景:有研究表明,CD4+、干扰素γ/诱导型一氧化氮合酶/一氧化氮通路与重症肌无力的发生密切相关。目的:探讨CD4+T细胞与干扰素γ/诱导型一氧化氮合酶/一氧化氮通路在脐带间充质干细胞移植治疗重症肌无力中的作用机制。方法:建立重症肌无力大鼠模型,并进行脐带间充质干细胞经静脉移植治疗,同时设立对照组。流式细胞术检测移植后大鼠腋窝淋巴结细胞CD4+的表达,ELISA法检测其干扰素γ的表达,Griess试剂和比色法检测一氧化氮和一氧化氮合酶水平。结果与结论:移植1周后,移植组大鼠腋窝淋巴结的淋巴细胞CD4+的表达显著高于模型组(P<0.01),干扰素γ、一氧化氮及诱导型一氧化氮合酶水平显著低于模型组(P<0.01)。证实,脐带间充质干细胞移植可上调重症肌无力模型大鼠淋巴细胞CD4+的表达,并调节干扰素γ/诱导型一氧化氮合酶/一氧化氮通路,下调一氧化氮水平,以减轻机体的免疫损伤。BACKGROUND： Several studies have demonstrated that CD4＋ and interferon-γ （INF-γ）-inducible nitric oxide synthane （iNOS）-nitric oxide （NO） pathway is closely related to occurrence of myasthenia gravis. OBJECTIVE： To investigate the mechanism underlying CD4＋ and IFN-γ-iNOS-NO pathway in treatment of myasthenia gravis by transplantation of umbilical cord mesenchymal stem cells. METHODS： Rat models of myasthenia gravis were treated by intravenous transplantation of umbilical cord mesenchymal stem cells. At the same time, a control group was set. The expression of CD4＋ on lymphocytes was tested by flow cytometry, the level of IFN-γ, iNOS and NO by ELISA, colorimetry and Griess, respectively. RESULTS AND CONCLUSION： At 1 week after transplantation, the expression of CD4＋ in the transplantation group was significantly higher （P 〈 0.01）, while the level of IFN-γ, iNOS and NO was significantly lower, than in the model group （P 〈 0.01）. These findings suggest that transplantation of umbilical cord mesenchymal stem cells can up-regulate the expression of CD4＋ in the lymphocytes in the transplantation group to adjust IFN-γ-iNOS-NO pathway, down-regulate NO level so as to alleviate immune injuries.

关 键 词：干扰素γ/诱导型一氧化氮合酶/一氧化氮通路 脐带间充质干细胞 重症肌无力 CD4+ 干细胞 

分 类 号：R394.2[医药卫生—医学遗传学]