骨髓腔注射骨髓间充质干细胞防治模拟失重大鼠的骨质疏松  

Preventive and therapeutic effects of intra-bone marrow cavity injection of bone mesenchymal stem cells on osteoporosis in rats subjected to simulated weightlessness

作　　者：吴礼凤[1] 孙平[2] 麦燕兴[1] 刘海明[1] 徐艳花[1] 杨锐[1] 黄震[3] 

机构地区：[1]南方医科大学附属珠江医院内分泌科,广东省广州市510282 [2]广东药学院第一附属医院骨质疏松科,广东省广州市510080 [3]南方医科大学附属珠江医院外科学教研室,广东省广州市510282

出　　处：《中国组织工程研究》2012年第19期3511-3514,共4页Chinese Journal of Tissue Engineering Research

摘　　要：背景:模拟失重条件下自身骨髓间充质干细胞的增殖受到抑制,并且向成骨细胞分化的能力减弱,造成骨量减少与骨微结构的破坏,最终导致骨质疏松。目的:观察同种异体骨髓间充质干细胞骨髓腔注射对模拟失重大鼠胫骨骨密度和骨组织微结构的影响。方法:将雄性SD大鼠随机分为自由活动对照组、尾吊模拟失重组、细胞治疗组(尾吊模拟失重同时给予双侧胫骨骨髓腔注射成骨诱导的同种异体BMSCs细胞)。结果与结论:与自由活动对照组相比,尾吊模拟失重组胫骨骨密度、骨小梁面积百分比、骨小梁数量和厚度均显著降低(P<0.01),骨小梁分离度显著增加(P<0.01)。与尾吊模拟失重组相比,细胞治疗组中胫骨骨密度、骨小梁面积百分比、骨小梁数量和厚度均显著增加(P<0.01),骨小梁分离度显著降低(P<0.01)。说明骨髓腔注射能够增加模拟失重大鼠骨密度,改善骨超微结构,减缓骨量丢失,有效防治骨质疏松。BACKGROUND： Simulated weightlessness can inhibit the proliferation of bone marrow mesenchymal stem cells and their differentiation towards osteoblasts, which leads to osteoporosis characterized by low bone mass and microarchitectural deterioration of bone tissue. OBJECTIVE： To study the effects of intra-bone marrow cavity injection of allogeneic bone mesenchymal stem cells （BMSCs） on the bone mineral density and microarchitecture of the tibias in rats subjected to simulated weightlessness. METHODS： Male SD rats were randomly divided into three groups： normal control group, tail-suspended simulated weightlessness group, and treatment group. The bilateral tibias were subjected to intra-bone marrow cavity injection of allogeneic BMSCs while giving the tail-suspended simulated weightlessness. RESULTS AND CONCLUSION： Compared with the normal control group, the bone mineral density, percentage of trabecular area, trabecular number and trabecular thickness were decreased in the tail-suspended simulated weightlessness group （P 〈 0.01）, while the trabecular separation was increased significantly （P 〈 0.01）. Compared with the tail-suspended simulated weightlessness group, the bone mineral density, percentage of trabecular area, trabecular number and trabecular thickness were increased in the treatment group （P 〈 0.01）, while the trabecular separation was decreased obviously （P 〈 0.01）. The intra-bone marrow cavity injection can increase the bone mineral density of rats subjected to simulated weightlessness, improve the bone ultrastructure, slow down bone loss, and have beneficial preventive and therapeutic effects on osteoporosis..

关 键 词：模拟失重 骨质疏松 骨髓间充质干细胞 骨密度 骨组织形态计量学 

分 类 号：R394.2[医药卫生—医学遗传学]