一种可用活体荧光成像技术检测的前列腺原位肿瘤模型的建立  

Establishment of animal model of orthotopic prostate tumor monitored by in vivo fluorescence imaging

作　　者：刘旭东[1] 曾津[1] 杨林[1] 徐珊[1,2] 张涛[3] 谢宏俊[1] 马振坤[1] 王新阳[1,2] 贺大林[1,2] 

机构地区：[1]西安交通大学医学院第一附属医院泌尿外科,陕西西安710061 [2]西安交通大学医学院第一附属医院环境和疾病相关基因教育部重点实验室,陕西西安710061 [3]山东大学附属省立医院泌尿外科,山东济南250021

出　　处：《现代泌尿外科杂志》2012年第3期225-228,共4页Journal of Modern Urology

基　　金：国家自然科学基金(No.81001147)

摘　　要：目的建立可用活体荧光成像技术动态观察前列腺原位肿瘤发生及生长的裸鼠模型。方法 10只BALB/c裸鼠前列腺背侧包膜内注入携带红色荧光蛋白(RFP)的前列腺癌PC-3细胞(PR7细胞)悬液20μL,第2、4、6、8周分别用非侵入式活体分子影像系统观察前列腺原位肿瘤发生及其转移情况。尸检取前列腺原位肿瘤、肺、肝、肾、股骨、盆腔淋巴结等组织,制成冰冻切片,通过荧光显微镜下观察及HE染色等方法分别检测肿瘤发生及有无转移等情况。结果第2周开始可用非侵入式活体分子影像系统检测到前列腺原位肿瘤形成,第4周开始可于裸鼠下腹部触及原位肿瘤,第6～8周裸鼠逐渐出现恶液质。荧光信号随原位肿瘤长大而增强,但并未见明显转移灶。尸检也未发现转移灶。结论前列腺包膜内注入携带RFP的前列腺癌PR7细胞可成功建立可用活体荧光成像技术监测的前列腺原位肿瘤模型,该模型可作为研究前列腺肿瘤发生发展及治疗方式的较理想的动物模型。Objective To establish an animal model of orthotopic prostate tumor in nude mice which can be monitored by in vivo fluorescence imaging.Methods 20 μL suspension of prostate cancer PR7（prostate cancer PC-3 cell which expresses red fluorescent protein stably） cell lines was injected into prostate capsule of 10 nude mice.The orthotopic and metastatic tumor were monitored with in vivo imaging technology at the end of 2nd,4th,6th and 8th week.Frozen sections of orthotopic tumor,lung,liver,kidney,adrenal gland,femur and lymph node were cut,placed on slides,and observed under a fluorescence microscope.Sections embedded in paraffin were stained with hematoxylin-eosin for histological examination.Results Orthotopic tumor could be detected by in vivo imaging at the end of the 2nd week.Tumor could be palpated outside the body from the 4th week.Mice developed dyscrasia from the 6th to 8th week.The fluorescence signal became stronger as the tumor size increased.However,no metastasis was detected.Conclusions Animal model of orthotopic prostate cancer monitored by in vivo fluorescence imaging is established successfully by intracapsularly injecting prostate cancer PR7 cells carrying red fluorescent protein（RFP）,which can serve as an ideal model for the study of the development of prostate cancer.

关 键 词：红色荧光蛋白 活体荧光成像 前列腺肿瘤 原位肿瘤 裸鼠 

分 类 号：R737.25[医药卫生—肿瘤]