HGF对局灶性脑缺血/再灌注大鼠脑含水量、MPO活性及TNF-α、IL-10的影响  

Effect of HGF on cerebral water content,activity of MPO,TNF-α and IL-10 in rats subjected to focal cerebral ischemia/reperfusion

作　　者：贺芳[1] 孙小娅[1] 向敏[2] 李畅[3] 

机构地区：[1]苏州卫生职业技术学院基础医学教研室,江苏苏州215009 [2]苏州卫生职业技术学院检验药学系,江苏苏州215009 [3]苏州大学附属第一医院心胸外科,江苏苏州215006

出　　处：《暨南大学学报（自然科学与医学版）》2012年第2期156-160,共5页Journal of Jinan University(Natural Science & Medicine Edition)

基　　金：江苏省卫生厅医学科研项目(H200966)

摘　　要：目的:观察HGF对脑缺血/再灌注(I/R)大鼠脑含水量、MPO活性及TNF-α、IL-10的影响。方法:SD大鼠随机分为5组:对照组;脑I/R组;实验Ⅰ、Ⅱ、Ⅲ组,分别用质量分数为15、30、60μg/kg HGF处理。线栓法制备大鼠大脑中动脉闭塞模型,缺血1.5 h再灌注24 h后,测定脑含水量、MPO活性,检测TNF-α、IL-10含量及mRNA水平的表达。结果:I/R大鼠脑含水量增加,MPO活性升高,TNF-α、IL-10含量及mRNA表达上升。不同质量分数HGF处理均能减少I/R大鼠脑含水量,降低MPO活性,下调TNF-α含量和mRNA水平,上调IL-10含量及mRNA表达。结论:促进抗炎症因子IL-10的表达、抑制促炎症因子TNF-α的表达可能是HGF减轻缺血性脑损伤炎症反应的机制之一。Aim：To investigate the effect of hepatocyte growth factor（HGF） on cerebral water content,activity of MPO,levels of TNF-α and IL-10 in rats after to focal cerebral ischemia/reperfusion（I/R）.Methods： Sprague-Dawley rats were randomly divided into five groups as： control group;I/R group;experimental groupⅠ,Ⅱ,Ⅲ,which were respectively treated with HGF of 15,30,60μg/kg HGF.A model of middle cerebral artery occlusion（MCAO） in rats was performed.After a transient MCAO of 1.5 h followed by reperfusion of 24 h,cerebral water levels,activity of MPO and content of TNF-α,IL-10 were measured.RT-PCR was conducted to determine the level of TNF-α and IL-10 mRNA.Results： I/R significantly increased cerebral water content,activity of MPO and content of TNF-α,IL-10 in brain tissue.The expression of TNF-α and IL-10 mRNA in brain tissue were remarkably increased.Treatment with HGF of different mass fraction decreased cerebral water content,activity of MPO,the mRNA level of TNF-α.While the mRNA level of IL-10 were increased.Conclusion：HGF can reduce the inflammatory response in focal cerebral ischemia/reperfusion injury.It may be related to promote the expression of anti-inflammatory factor IL-10,and decrease expression of pro-inflammatory factor TNF-α.

关 键 词：肝细胞生长因子 脑缺血 再灌注 大鼠 

分 类 号：R743[医药卫生—神经病学与精神病学]