Effects of Echinacoside on Histio-central Levels of Active Mass in Middle Cerebral Artery Occlusion Rats  

作　　者：WEI Li Li CHEN Hong JIANG Yong TU Peng Fei ZHONG Ming DU Juan LIU Fei WANG Lei LIU Chun Yang 

机构地区：[1]Ministry of Education Key Laboratory of Xinjiang Phytomedicine Resources, Shihezi University, Shihezi 832002, Xinjiang, China [2]Department of Pharmacology, College of Pharmacy, Shihezi University, Shihezl 832002, Xinjiang, China [3]Peking University Modern Research Center for Traditional Chinese Medicine, Beijing 100083, China

出　　处：《Biomedical and Environmental Sciences》2012年第2期238-244,共7页生物医学与环境科学（英文版）

基　　金：supported by the National Natural Science Foundation of China (No.30560171,No.30860334)

摘　　要：Objective To investigate the effects of echinacoside on the extracellular striatal levels of norepinephrine（NE）,dopamine（DA）,homovanillic acid（HVA）,3,4-dihydroxyphenylethanoid acid（DOPAC）,5-hydroxyindoleacetic acid（HIAA）,and 5-hydroxytryptamine（5-HT） in middle cerebral artery occlusion（MCAO rats.Methods The middle cerebral artery was occluded in male Sprague-Dawley rats.Three days later microdialysis probes were placed into the right striatum of MCAO rat brains and the brains were perfused with Ringer＇s solution at a rate of 1.5 μL/min.Cerebral microdialysates were collected every 30 minutes from awake and freely moving rats before assaying for NE,DA,HVA,DOPAC,HIAA,and 5-HT levels by reverse phase HPLC with electrochemistry.Results Three days after MCAO,the extracellular striatal levels of NE,DA,DOPAC,HIAA,HVA,and 5-HT of the MCAO rats increased significantly（at least P0.05 vs.control）.However,simultaneous treatment with echinacoside（30.0 or 15.0 mg/kg） attenuated these increases（at least P0.05 vs.non-treated model rats）.Conclusion These results imply that echinacoside may protect striatal dopa minergic neurons from the injury induced by MCAO and may help prevent and treat cerebral ischemic diseases.Objective To investigate the effects of echinacoside on the extracellular striatal levels of norepinephrine（NE）,dopamine（DA）,homovanillic acid（HVA）,3,4-dihydroxyphenylethanoid acid（DOPAC）,5-hydroxyindoleacetic acid（HIAA）,and 5-hydroxytryptamine（5-HT） in middle cerebral artery occlusion（MCAO rats.Methods The middle cerebral artery was occluded in male Sprague-Dawley rats.Three days later microdialysis probes were placed into the right striatum of MCAO rat brains and the brains were perfused with Ringer＇s solution at a rate of 1.5 μL/min.Cerebral microdialysates were collected every 30 minutes from awake and freely moving rats before assaying for NE,DA,HVA,DOPAC,HIAA,and 5-HT levels by reverse phase HPLC with electrochemistry.Results Three days after MCAO,the extracellular striatal levels of NE,DA,DOPAC,HIAA,HVA,and 5-HT of the MCAO rats increased significantly（at least P0.05 vs.control）.However,simultaneous treatment with echinacoside（30.0 or 15.0 mg/kg） attenuated these increases（at least P0.05 vs.non-treated model rats）.Conclusion These results imply that echinacoside may protect striatal dopa minergic neurons from the injury induced by MCAO and may help prevent and treat cerebral ischemic diseases.

关 键 词：ECHINACOSIDE Norepinephrine （NE） dopamine （DA） homovanillic acid （HVA） 3 4-dihydroxyphenylethanoid acid （DOPAC） 5-hydroxyindoleacetic acid （HIAA） 5-hydroxytryptamine （5-HT） Middle cerebral artery occlusion（MCAO） Brain microdialysis RATS 

分 类 号：Q95-331[生物学—动物学] S68[农业科学—观赏园艺]