水溶性黑柄炭角菌肽的体外抗氧化活性  

Anti-oxidative activity of water-soluble peptides from Xylaria nigripes in vitro

作　　者：翁榕安[1] 胡劲松[1] 翁诗玉[1] 

机构地区：[1]湖南师范大学,湖南长沙410081

出　　处：《湖南中医药大学学报》2012年第3期10-13,共4页Journal of Hunan University of Chinese Medicine

基　　金：湖南省自然科学基金资助项目(10JJ3039)

摘　　要：目的研究水溶性黑柄炭角菌肽的体外抗氧化活性。方法通过测定水溶性黑柄炭角菌肽对邻苯三酚自氧化产生的超氧阴离子、Fenton反应产生的羟自由基、DPPH自由基的清除率,还原能力及抑制脂质体过氧化,全面考察水溶性黑柄炭角菌肽的体外抗氧化能力。结果水溶性黑柄炭角菌肽能明显降低邻苯三酚自氧化速率,对羟自由基有极强的清除能力,对DPPH自由基清除率随浓度增加而增加,其IC50分别为10、0.49 mg/mL和16.3μg/mL;进一步研究表明,黑柄炭角菌肽具有一定的还原力且与浓度呈正比例关系,同时也能抑制脂质体的过氧化反应。结论水溶性黑柄炭角菌肽在体外具有明显的抗氧化作用。Objective To study anti-oxidative activity of water-soluble peptides from Xylaria nigripes（XNP） in vitro.Methods The anti-oxidative activity of water-soluble peptides from XNP were studied entirely by measuring scavenging rate of water-soluble peptides from XNP on super-oxide anion generated by pyrogallol autoxidation,hydroxyl free radical in Fenton reaction and DPPH free radical,and their reducing power and inhabiting peroxidation of liposomes ability.Results Water-soluble peptides from XNP could significantly reduce pyrogallol autoxidation rate,had highly scavenging capacity of hydroxyl free radical,and had dose dependent in scavenging DPPH free radical scavenging.Their IC50 were 10,0.49 mg/mL and 16.3 ug/mL respectively.Futher research showed that water-soluble peptides from XNP had certain reducing power increased as concentration increased,they also could inhibit the peroxidation of liposomes.Conclusion Water-soluble peptides from XNP was a good anti-oxidant in vitro test.

关 键 词：黑柄炭角菌 肽 抗氧化 体外试验 

分 类 号：R285.5[医药卫生—中药学]