急性化脓性扁桃体炎证候层次与Th1/Th2漂移相关性研究  

Corelation of syndrome stage and Th1/Th2 shift in acute suppurative tonsillitis

作　　者：刘旺华[1] 刘建新[2] 周小青[1] 谢静[3] 杨静宜[3] 

机构地区：[1]湖南中医药大学中医诊断学重点学科中医诊断学精品课程中医诊断学教学团队中医诊断学实验室病理生理实验室中医病症实验室,湖南长沙410007 [2]湖南中医药大学中医临床基础学科,湖南长沙410007 [3]湖南中医药大学第一附属医院,湖南长沙410007

出　　处：《湖南中医药大学学报》2012年第3期49-52,共4页Journal of Hunan University of Chinese Medicine

基　　金：湖南省教育厅科研项目(08C621;10A093);湖南省高校创新平台开放基金项目(101C047)

摘　　要：目的研究急性化脓性扁桃体炎患者不同证候层次外周血Th1/Th2漂移变化。方法通过流式细胞检测技术,对急性化脓性扁桃体炎患者卫分证、气分证、营分证和正常人外周血Th1/Th2细胞进行检测,采用ELISA法测定血清细胞因子IL-4和IFN-γ水平,并比较IFN-γ/IL-4比值。结果与正常对照组比较,急性化脓性扁桃体炎卫分证组Th1、IFN-γ、Th1/Th2、IFN-γ/IL-4比值显著升高,差异有统计学意义（P〈0.05）;气分证组Th1、Th2、IFN-γ、IL-4明显升高,差异有统计学意义（P〈0.05~0.01）;营分证组Th1、Th2、IFN-γ、IL-4继续明显升高,Th1/Th2比值却明显降低,差异有统计学意义（P〈0.05~0.01）。与卫分证组比较,气分证组Th1、Th2、IFN-γ、IL-4显著升高,Th1/Th2、IFN-γ/IL-4比值明显降低,差异有统计学意义（P〈0.05~0.01）;营分证组Th2、IL-4继续明显升高,Th1/Th2、IFN-γ/IL-4比值明显降低,差异有统计学意义（P〈0.01）。与气分证组比较,营分证组Th1、IFN-γ水平、Th1/Th2、IFN-γ/IL-4比值显著降低,Th2、IL-4显著升高,差异有统计学意义（P〈0.05~0.01）。结论急性化脓性扁桃体炎卫分证、气分证、营分证证候层次变化与Th1/Th2漂移存在相关性。Objective To study the changes of Th1/Th2 shift in different syndrome stage of acute suppurative tonsillitis.Methods Flow cytometry was used to detect the Th1/Th2 cell in peripheral blood of the patients of weifen zheng,qifen zheng,yingfen zheng and healthy peop1e,the expressions of IL-4 and IFN-γ were detected by ELISA,and the ratio of IFN-γ/IL-4 is measured.Results Compared with normal group,the number of Th1 and the content of IFN-γ and the ratio of Th1/Th2 and IFN-γ/IL-4 of weifen zheng group increased significantly（P0.05）,the number of Th1 and Th2 and the contents of IFN-γ and IL-4 of qifen zheng group improved markedly（P0.05~0.01）,the numbers of Th1 and Th2 and the content of IFN-γ and IL-4 of yingfen zheng group improved markedly continuously while the ratio of Th1/Th2 decreased obviously（P0.05~0.01）.Compared with weifen zheng group,the numbers of Th1 and Th2 and contents of IFN-γ and IL-4 increased distinctly while the ratio of Th1/Th2 and IFN-γ/IL-4 decreased markedly in qifen zheng group（P0.05~0.01）,the number of Th2 and the contain of IL-4 of yingfen zheng group improved markedly continuously,while the ratio of Th1/Th2？IFN-γ/IL-4 decreased obviously（P0.01）.Compared with qifen zheng group,the number of Th1 and the content of IFN-γ and the ratios of Th1/Th2 and IFN-γ/IL-4 decreased markedly while the number of Th2 and the content of IL-4 increased distinctly in yingfen zheng group（P0.05~0.01）.Conclusion The drifting of Th1/Th2 may play important role during the different syndrome stage in acute suppurative tonsillitis.

关 键 词：扁桃体炎 T淋巴细胞亚群 TH1/TH2类细胞因子 流式细胞术 

分 类 号：R743[医药卫生—神经病学与精神病学]