乌司他汀对兔脑出血脑保护作用的实验研究  

Effect of Ulinastatin on Cerebral Protection during Rabbit Intracerebral Hemorrhage

作　　者：许莉[1] 侯群[1] 佟丽妍[2] 

机构地区：[1]浙江中医药大学第一临床医学院,杭州310053 [2]杭州市中医院

出　　处：《浙江中西医结合杂志》2012年第5期339-341,共3页Zhejiang Journal of Integrated Traditional Chinese and Western Medicine

基　　金：天普研究基金项目(No.01200919)

摘　　要：目的:通过观察脑出血兔血清可溶性血栓调节蛋白(STM)、血管假血友病因子(vWF)、一氧化氮(NO)含量变化,探讨乌司他汀的脑保护作用。方法:40只新西兰大白兔随机分为正常对照组、模型组、乌司他汀1天组和乌司他汀3天组,每组10只。用自体血法制备兔脑出血模型,应用Ellisa法测定各组兔血清STM、vWF、NO含量。结果:与正常对照组比较,模型组STM、vWF和NO含量显著增高(P<0.05)。乌司他汀1天组、3天组的STM、vWF和NO均低于模型组(P<0.05),乌司他汀3天组STM、vWF和NO含量与乌司他汀1天组比较,差异无统计学意义(P>0.05)。结论:乌司他汀抑制STM、vWF和NO生成,从而减轻脑出血兔脑细胞的损伤,具有脑保护作用。Objective：To explore the effect of ulinastatin on the contents of soluble thrombomodulin（STM）,von Willebrand factor（vWF）,and nitric oxide（NO） in rabbits with intracerebral hemorrhage（ICH）.Methods： Rabbit ICH model was prepared with autologous blood.Forty New Zealand white rabbits were randomly divided into 4 groups of 10 each,including normal control group,sham operation group,ulinastatin 1-day group,and ulinastatin 3-day group.A double-antibody sandwich enzyme-linked immunosorbent assay（ELISA） was used to assess the levels of rabbit STM,vWF,and NO.Results： Compared with the normal control group,the levels of STM,vWF,and NO in the sham group were significantly higher（P〈0.05）.Compared with the sham operation group,the STM,vWF,and NO levels were lower in the ulinastatin 1-day and 3-day groups（P〈0.05）.No significant difference in these parameters was noted between the ulinastatin 1-day group and the ulinastatin 3-day group（P〈0.05）.Conclusion： Ulinastatin can cut down the generation of STM,vWF,and NO to less cerebral damage during rabbit ICH.

关 键 词：新西兰兔 脑出血 乌司他汀 STM VWF NO 

分 类 号：R965[医药卫生—药理学]