Cryptophycin诱导肾癌细胞凋亡的研究  

Apoptosis Induced by Cryptophycin in Renal Cancer Cell Line

作　　者：周鹤同[1] 郭震华[2] 苏长青[3] 严春寅[2] 邵竹平[1] 李东[1] 

机构地区：[1]南京江北人民医院,210048 [2]苏州医学院第一附属医院,215006 [3]解放军第81医院全军肿瘤研究中心,210002

出　　处：《实用癌症杂志》2000年第3期235-237,共3页The Practical Journal of Cancer

摘　　要：目的　探讨诱导肾癌细胞凋亡的新方法及其机制。方法　采用HE染色、荧光染色、琼脂糖电泳等方法 ,分别对Cryptophycin(CPP)不同的药物浓度 ( 0 ,1,5 ,10 ,5 0 pmol/L)和不同培养时间段 ( 4～ 2 4h)所诱导的凋亡细胞形态、出现时间和密度比例进行观察和比较。结果　在光镜下能观察到CPP诱导的肾癌细胞凋亡的典型形态 ,用AO/EB荧光染色能区分出 4种不同细胞 ,DNA电泳证实有DNA裂解的现象。CPP在 10 pmol/L水平诱导细胞凋亡比率较高 ,作用 12h已基本达到凋亡的较高水平。结论　CPP可诱导肾癌细胞凋亡的发生 ,药效敏感。Objective To study a new way of inducing renal cancer cell apoptosis.Methods The renal cancer cell apoptosis induced by cryptophycin(CPP) in different concentration and different culture time was observed by HE staining,fluorescent staining and DNA electrophoresis.Results The typical characteristic of renal cancer cell apoptosis could be observed under light microscope.Four special cells could be distinguished by AO/EB fluorescent staining.The phenomenon of DNA degradation was proved by DNA electrophoresis.CPP could induce high proportion of apoptosis at 10 pmol/L drug concentration and in a culture time of 12 h.Conclusion CPP can induce renal cancer cell apoptosis sensitively.

关 键 词：肾肿瘤 细胞凋亡 Cryptophycin 

分 类 号：R737.11[医药卫生—肿瘤]