肺癌组织中p53、K-ras、p15、p16基因的改变  

Changes of p53、K-ras and p15、p16 Gene in the Lung Cancer Tissues

作　　者：吴小军[1] 杨炯[1] 丁续红 胡克 李清泉[1] 

机构地区：[1]湖北医科大学附一院呼吸内科,430060

出　　处：《实用癌症杂志》2000年第3期255-258,共4页The Practical Journal of Cancer

基　　金：湖北省"95"攻关项目! (编号 961P1 80 1 )

摘　　要：目的　探讨p5 3、K ras、p15、p16基因在肺癌组织中改变情况及多基因联合检测在肺癌诊断中的价值。 方法　对 5 9例肺癌组织和 14例肺部良性组织 ,应用PCR SSCP 银染法检测 p5 3基因第 5～ 8外显子突变情况 ;应用PCR RFLP法对K ras基因突变进行检测 ;并应用PCR法检测 p15、p16基因纯合缺失情况。 结果　肺癌组织中 p5 3、p15、p16基因改变频率分别为 3 7.3 %、11.9%、2 3 .8% ,而在肺部良性组织中只有 1例 p16基因阳性。在 2 5例肺腺癌中 ,K ras基因突变率为 48.0 % ,其它类型肺癌中突变率仅为 8.8% ,而 14例良性组织中未检测出K ras基因突变。另外 ,p5 3基因和K ras基因突变与吸烟存在着密切关系 ,即吸烟者出现突变的频率 ( 4 8.7% ,68.5 % )明显高于非吸烟者 ( 15 .0 % ,11.1% ) ,P <0 .0 1。 4种基因联合检测敏感度为 78.0 % ,明显高于单一基因 (以阳性率最高的 p5 3为例 )的敏感度 3 7.3 %。 结论　p5 3基因、K ras基因和 p16基因在肺癌组织中改变率相对较高 ;多基因的联合检测有助于肺癌的早期诊断和筛查。Objective To assess the changes of p53、K ras、p15、p16 gene and the diagnostic value of multiple genes detection in lung cancer.Methods In 59 cases of lung cancer and 14 cases of benign respiratory diseases,the mutations of 5 8 exon of p53 gene were detected by PCR SSCP AgNO 3 staining method,the mutations of first exon of K ras gene by PCR RFLP technique,the homozygous deletion of p15 and p16 gene by PCR technique.Results The frequency of p53、p15、p16 alteration in lung cancer was 37.3% 、11.9%、23.8% respectively and only one case of benign disease showed p16 homozygous deletion.The mutation rate of K ras gene was 48.0% in adenocarcinomas.No K ras gene mutations was detected in the specimens of benign respiratory diseases.The mutation rate of p53 and K ras in smokers(48.7%,68.5%) were higher than non smokers(15.0%,11.1%), P <0.01.The sensitivity of four markers combined detection(78.0%) was obviously higher than that of any single gene detection.Conclusions The changes of p53,K-ras and p16 are common in lung cancer and combination detection of four marker is better than single marker for the diagnosis of lung cancer.

关 键 词：肺癌 诊断 P53基因 K-RAS基因 P15基因 P16基因 

分 类 号：R734.202[医药卫生—肿瘤]