热疗联合顺铂对小细胞肺癌NCI-H446细胞凋亡的影响及机制探讨  

Effect and mechanism of hyperthermia and DDP on apoptosis of small cell lung cancer cell line NCI-H446

作　　者：黄波[1] 王晓东[1] 郎贤平[1] 

机构地区：[1]辽宁医学院附属第一医院,辽宁锦州121000

出　　处：《山东医药》2012年第18期1-3,共3页Shandong Medical Journal

基　　金：辽宁省教育厅科研基金资助项目(L2010302);辽宁医学院附属第一医院院内课题项目(2010C09)

摘　　要：目的观察热疗联合顺铂对小细胞肺癌NCI-H446细胞凋亡的影响,并探讨其可能的机制。方法将对数生长期的NCI-H446细胞随机分为A、B、C、D组,A组常规培养不进行特殊处理,B组将细胞消化后放置离心管中在42℃水浴中加热2 h,C组加入含2μg/mL顺铂的培养液4μg/孔,D组先经过水浴处理后加入顺铂。继续培养72 h后,采用流式细胞仪测算各组细胞凋亡率,采用Western blot法检测各组细胞中的Bcl-2、Bax。结果 NCI-H446细胞凋亡率A组为4.25%±0.56%、B组为8.12%±0.64%、C组为10.02%±0.82%、D组为12.12%±0.49%,B、C、D组细胞凋亡率与A组比较,P均<0.05;D组与B、C组比较,P均<0.05。NCI-H446细胞中Bcl-2、Bax相对表达量A组分别为0.89±0.002 8、0.18±0.001 8,B组分别为0.61±0.004 2、0.32±0.002 0,C组分别为0.30±0.003 6、0.48±0.001 4,D组分别为0.21±0.001 7、0.64±0.002 5。B、C、D组与A组比较,P均<0.05;D组与B、C组比较,P均<0.05。结论热疗联合顺铂可诱导小细胞肺癌NCI-H446细胞凋亡,其机制与降低细胞中Bcl-2表达、升高Bax表达有关。Objective To observe the effect of hyperthermia and cisplatin on apoptosis of small cell lung cancer cell line NCI-H446,and explore its possible mechanism. Methods Exponentially growing NCI-H446 cells were divided into control group（group A）, hyperthermia treatment group（group B）, cisplatin treatment group （group C）and combined hyperthermia and cisplatin therapy group（ group D）. After the digestion, the cells in group B were placed the centrifuge tube, heated in 42 ℃ water bath for 2 h ; the cells in group C were cultured with 2 μg/mL cisplatin, 4 μg per well ; the cells in group D were placed water bath before cisplatin, the cells in group A were cultured without special treatment. After cultured for 72 h, flow cytometry was used to detect apoptosis rate and western blot was used to detect the changes of apopto- sis-related gene Bcl-2, Bax in each group. Results The apoptosis rate of the cells was 8.12% ± 0.64% in group B, 10.02%±0.82% in group C, 12.12% ±0.49% in group D, 4.25%±0.56% in group A. The cell apoptosis rate of group B, C, D were higher than group A, all P 〈 0. 05 ; group D was higher than group B and C, all P 〈 0.05. The Bcl-2 and Bax expression in group A were 0.89±0. 002 8, 0.18± 0. 001 8 ; group B were 0.61±0.004 2, 0.32 ±0. 002 0 ; group C were 0.30±0. 003 6, 0.48±0. 001 4 ; group D were 0.21±0.001 7, 0.64 ±0.002 5. The Bcl-2 and Bax expression of group B, C, D were higher than group A, all P 〈 0.05 ; group D was higher than group B, C, all P 〈 0.05. Conclusion Hyperthermia combined with cisplatin can induce small cell lung cancer NCI-H446 cell apoptosis, its mechanism may related with reducing the Bcl-2 expression and increasing Bax expression.

关 键 词：热能疗法 顺铂 细胞凋亡 B细胞淋巴瘤细胞因子2 人Bcl-2相关X蛋白 小细胞肺癌 肺肿瘤 

分 类 号：R734.2[医药卫生—肿瘤] R730.5[医药卫生—临床医学]