IL-10和IL-12在结直肠癌肝转移中作用的临床研究  

The Clinical Study on the Effect of IL-10 and IL-12 in postoperative Colorectal Cancer with Liver Metastasis

作　　者：秦建民[1] 盛霞[1] 杨林[1] 撒忠秋[1] 徐夏君[1] 李琦[1] 殷佩浩[1] 张敏[1] 陈腾[1] 曹伟家[1] 

机构地区：[1]上海中医药大学附属普陀医院普外科,上海200062

出　　处：《肝胆外科杂志》2012年第2期116-119,共4页Journal of Hepatobiliary Surgery

基　　金：上海市卫生局科研基金资助(编号07-59)

摘　　要：目的探讨IL-10、IL-12在结直肠癌肝转移中作用。方法分别收集自2008年1月到2010年6月健康成年人、结直肠癌术前、术后和术后肝转移患者外周血,采用ELISA法测定外周血清IL-10、IL-12水平,采用FACS Calibur3.0流式细胞仪测定T淋巴细胞及其亚群比例,进行各组结果统计学分析。结果结直肠癌术后肝转移患者外周血清IL-10水平显著高于健康成年人、结直肠癌术后患者(P<0.05),结直肠癌术后患者外周血清IL-10水平显著低于术前患者(P<0.05);结直肠癌术前、术后和术后肝转移患者外周血清IL-12水平显著低于健康成年人,结直肠癌术前、术后患者之间以及术后和术后肝转移患者之间外周血清IL-12水平存在统计学差异(P<0.05);结直肠癌术后肝转移患者外周血T淋巴细胞总数及Th细胞比例数显著低于健康成年人(P<0.05),Ts细胞比例数显著高于健康成年人(P<0.05)。NK细胞在结直肠癌术后肝转移患者显著低于健康成年人(P<0.05)。结论不同临床时期结直肠癌患者存在细胞免疫或体液免疫严重障碍,纠正机体细胞因子和免疫状态紊乱,不仅能够改善机体免疫功能,可能有助于控制结直肠癌的发生、发展。Objective To observe the effect of IL-10 and IL-12 in postoperative colorectal cancer with liver metastasis.Methods The samples of peripheral blood were respectively collected form the healthy adults,patients of preoperative and postoperative coloreatal cancer,patient of postoperative coloreatal cancer with liver metastasis since Jan.2008 to Jun.2010.The level of IL-10 and IL-12 was detected with ELISA method,The T lymphocyte and their subgroups were detected with FACS Calibur 3.0 flow cytometry,the results were statistically analyzed.Results The level of IL-10 in postoperative coloreatal cancer with liver metastasis was significantly higher than that of healthy adult and postoperative coloreatal cancer（P0.05）,the level of IL-10 in postoperative coloreatal cancer was significantly lower than that of preoperative coloreatal cancer（P0.05）.The level of IL-12 in preoperative,post operative coloreatal cancer and postoperative coloreatal cancer with liver metastasis was significantly lower than that of healthy adults（P0.05）,the levelof IL-12 between preoperative and postoperative coloreatal cancer as well as between postoperative coloreatal cancer and postoperative coloreatal cancer with liver metastasis was significant difference（P0.05）.The number of T lymphocyte and Th cell in postoperative coloreatal cancer with liver metastasis was significantly fewer than that of healthy adult（P0.05）,the number of Ts cell in postoperative coloreatal cancer with liver metastasis was significantly more than that of healthy adult（P0.05）.The number of NK cell in post-operative coloreatal cancer with liver metastasis was significantly fewer than that of healthy adult（P0.05）.Conclusions Cell and/orhumoral immunity in colorectal cancer with different clinical stage occur serious disorder.It can improve the immune function of patients and decrease the occurrence and development of colorectal cancer by collecting the immune disorder of cell cytokines and immunestate in various patients with colorectal cancer.

关 键 词：结直肠癌 肝转移 细胞因子 作用 

分 类 号：R735.9[医药卫生—肿瘤]