天花粉凝集素的提取工艺及降糖活性研究  

Study on the Extraction Technology and Hypoglycemic Activity of Lectin from Trichosanthes kirilowi

作　　者：李琼[1] 叶小利[2] 曾红[1] 陈新[1] 李学刚[1,3] 

机构地区：[1]西南大学药学院,重庆400715 [2]西南大学生命科学学院,重庆400715 [3]重庆市药效学评价工程中心,重庆400715

出　　处：《中药材》2012年第3期475-479,共5页Journal of Chinese Medicinal Materials

基　　金：中央高校基本科研业务费(XDJK2011C051)

摘　　要：目的:从天花粉中提取凝集素并研究其降糖活性。方法:采用单因素及正交试验对各提取因素进行优化,确定最佳提取工艺参数;用硫酸铵分级沉淀得到凝集素粗品;以5%的A型人血细胞凝血效价为指标,测定其凝集活性;检测天花粉凝集素对人肝癌细胞HepG2的葡萄糖消耗量及四氧嘧啶糖尿病小鼠血糖的影响。结果:天花粉凝集素凝血效价为32;细胞试验和动物试验结果均显示天花粉凝集素表现出降糖活性。结论:天花粉凝集素最佳提取工艺为:缓冲液PBS,料液比1∶30,提取时间24 h,pH7.2,硫酸铵最佳饱和度70%,离心转速10 000 r/min;天花粉凝集素具有较强的降糖作用。Objective： To extract lectins from Trichosanthes kirilowi and study their hypoglycemic activity.Methods： The optimal extraction process included the following parameters were conformed by optimization analysis,lectins extracted from Trichosanthes kirilowi was achieved by ammonium sulfate precipitation;The agglutinate activity was determined by using the agglutination test with 5%human blood cells.Human hepatocarcinoma cell HepG2 and the alloxan-induced diabetic mice model were used to assess hypoglycemic activity of Lectin in Trichosanthes kirilowi.Results： The agglutination indexes of lectins extraction buffer were 32;The cell and mice tests indicated that the lectins exhibited hypoglycemic activity in the 70% saturation.Conclusion： The optimum extraction technology is as follows： extraction with PBS,the material-water ratio is 1∶30,the extraction time is 24h,while the concentration of sodium chloride is 0 mol/L and pH is 7.2.Precipitate lectins by ammonium sulfate in the 70% saturation,centrifugal speed is 10 000 r/min.Lectins extracted from Trichosanthes kirilowi exposes proper hypoglycemic activity.

关 键 词：天花粉 凝集素 提取工艺 降糖活性 

分 类 号：R286.1[医药卫生—中药学]