骨桥蛋白在不同种植方式种植义齿骨界面改建中的变化及意义  

Expression of osteopontin in the process of implant-bone interface remodeling influenced by submerged or nonsubmerged implant denture

作　　者：袁林[1] 童心[1] 李方舟[2] 曹凯华[1] 

机构地区：[1]广州医学院第一附属医院口腔科,510140 [2]中山市人民医院口腔科

出　　处：《实用口腔医学杂志》2012年第3期285-288,共4页Journal of Practical Stomatology

基　　金：广东省科技计划项目(编号:2008B030301246);广州市科技支撑计划项目(编号:2008Z1-E321)

摘　　要：目的:对比埋植型与非埋植型种植体在义齿修复受载后种植体-骨界面中骨桥蛋白表达变化的异同。方法:选用纯系动物Beagle犬8只,在其下颌分别植入埋植型与非埋植型种植体,采用固定金属全冠行种植义齿修复,分不同时期处死动物。采用免疫组化方法,对修复后不同时期埋植型与非埋植型种植体-骨界面骨桥蛋白进行动态观察,比较两者之间的异同。结果:免疫组化观察发现,种植义齿修复受载后,随着种植体周围骨组织发生改建,种植体-骨界面表达骨桥蛋白较未修复对照组明显增强。2周时界面骨桥蛋白有表达,但与对照组无差异;4周和8周时明显高于对照组,并于8周时达到高峰;12周时恢复到与对照组相近。埋植型和非埋植型种植体之间无显著差异。结论:埋植型和非埋植型种植体在义齿修复受载后,所承受的机械负荷均能经过种植体传递至周围骨组织,刺激种植体-骨界面骨桥蛋白表达。Objective： To study the expression of osteopontin（OPN） in the process of implant-bone interface remodeling after loading of submerged or nonsubmerged implant dentures.Methods： 8 adult beagle dogs were used to build the animal model.Submerged and nonsubmerged implants were implanted into the bilateral mandible respectively.Fixed metal full crown was used to carry out the submerged and nonsubmerged implant dentures.The beagle dogs were sacrificed by steps after loading.Immunohistochemistry was used to observe the expression of osteopontin in the implant-bone interface.Results： In the group after loading of implant denture,higher expression of OPN in the implant-bone interface was observed than in the unloading control.2 weeks after loading of implant denture,the expression of OPN in the interface was observed,but there was no statistical difference between experimental and control groups.4 and 8 weeks after loading,the expression of OPN in the loading groups was higher than that in the control group（P0.01）,OPN expression reached peak at 8 weeks.It came down to the level of the control group 12 weeks after loading.No significant difference of the OPN expression in the implant-bone interface was observed between the submerged and nonsubmerged implant dentures.Conclusion： The mechanical stress which transferred from implant to the bone tissue after loading of the submerged and nonsubmerged implant dentures can stimulate the expression of OPN in the implant-bone interface,promote the interface remodeling.

关 键 词：埋植型 非埋植型 种植体-骨界面 受载 改建 骨桥蛋白 

分 类 号：R783.6[医药卫生—口腔医学]