肥大细胞脱颗粒在牙周炎病理中的作用  

Pathophysiological significance of mast cell degranulation in periodontitis

作　　者：潘倩茹[1] 吕芳丽[2] 黄博[2] 陈莹[2] 刘曼[2] 黄世光[1] 

机构地区：[1]暨南大学医学院口腔医学系,广州510632 [2]广州中山大学医学院寄生虫教研室

出　　处：《实用口腔医学杂志》2012年第3期316-319,共4页Journal of Practical Stomatology

基　　金：国家自然科学基金项目(编号:81071387);广东省自然科学基金项目(编号:10151008901000006)

摘　　要：目的:探讨肥大细胞脱颗粒在牙周炎病理中的意义。方法:将自愿接受研究的受试者分为3组:轻度慢性牙周炎组(轻度组,17例)、重度慢性牙周炎组(重度组,18例)、正常对照组(15例)。取牙龈活检组织制作连续切片,HE染色在光镜下观察各组牙周组织的组织学改变;甲苯胺蓝染色法观察各组肥大细胞的数量及肥大细胞脱颗粒状况;天狼猩红染色法观察各组牙周组织纤维化的程度。结果:与正常对照组相比,各慢性牙周炎组的牙周组织中肥大细胞显著增多(P<0.01);重度组与轻度组相比较肥大细胞数量和脱颗粒率均明显升高(P<0.01),牙周组织纤维化程度更加严重(P<0.05)。结论:肥大细胞的募集、脱颗粒和纤维化程度与牙周炎的严重程度相关,提示肥大细胞可能在牙周炎的发病和疾病进程中起着重要的作用。Objective： To study the pathological significance of mast cell degranulation in human periodontitis.Methods： A total of 50 donors including 17 with mild chronic periodontitis,18 with advanced chronic periodontitis（n=18） and 15 of healthy subjects were involved in this study.Gingiva specimens were fixed in 10% buffered formalin,stained with hematoxylin and eosin for histopathologic observation,stained with toluidine blue for identification of mast cells and its degranulation,and stained with picric-sirius red for detecting fibrosis degree in periodontal tissues.Results： More mast cells with more degranulation were observed in advanced chronic periodontitis groups than in the other 2 groups（P0.01） and in mild periodontitis group than in healthy control group（P0.01）.More severe pathogenesis with stronger fibrosis was found in advanced chronic periodontitis group than in mild chronic periodontitis group（P0.05）.Conclusion： Mast cells and their degranulation may contribute to the progression of periodontal disease and severity of tissue fibrosis.

关 键 词：牙周炎 肥大细胞 脱颗粒 纤维化 

分 类 号：R781.4[医药卫生—口腔医学]