围手术期不同处理因素对SD大鼠肠道菌群的影响  

Effect of different perioperative treatments on gut flora in SD rats

作　　者：刘汉成[1] 张党政[1] 王东升[1] 王茂龙[1] 周岩冰[1] 

机构地区：[1]青岛大学医学院附属医院普通外科,266003

出　　处：《中华胃肠外科杂志》2012年第6期581-584,共4页Chinese Journal of Gastrointestinal Surgery

摘　　要：目的探讨围手术期不同处理因素对SD大鼠肠道菌群数量及比例的影响。方法根据围手术期不同处理因素，将SD大鼠48只分成对照组、抗生素组、肠道准备组、禁食组、抗生素-肠道准备组、抗生素-肠道准备一禁食组、肠道准备-手术处理-抗生素．术后早期进食组（术后早期进食组）及肠道准备-手术处理．抗生素一术后禁食组（术后禁食组）8组。每组6只．处死后取回盲部粪便．采用倍比稀释法分别涂在选择性培养基上，37℃恒温培育48h，对大肠杆菌、类杆菌、双歧杆菌和肠球菌计数。结果与对照组相比，抗生素组大鼠细菌总数、类杆菌、肠球菌和双歧杆菌计数均明显下降（P〈0．05），而大肠杆菌水平上升（P〈0．05），杆菌与球菌的比例（杆球比）升高（P〈O．05）：肠道准备组大鼠细菌总数、类杆菌、双歧杆菌和肠球菌计数均明显下降（P〈0．05），而大肠杆菌无明显变化（P〉O．05）。杆球比下降（P〈0．05）；禁食组细菌总数、类杆菌、肠球菌和双歧杆菌计数均明显降低（P〈0．05），大肠杆菌计数无明显改变（P〉0．05），杆球比下降（P〈0．05）。术后早期进食组与术后禁食组相比，大肠杆菌、肠球菌及细菌总数明显升高（P〈0．05），杆球比下降（P〈0．05）。结论抗生素、肠道准备及禁食均可影响SD大鼠肠道菌群数量和比例的改变，导致肠道菌群失调：术后早期进食对菌群失调有改善作用。Objective To study the effects of different perioperative treatments on the number and proportion of gut flora in SD rats. Methods Forty-eight SD rats were randomized into 8 groups including the control group, antibiotics group, bowel preparation group, fasting group, antibiotic-bowel preparation group, antibiotics-bowel preparation-fasting group, bowel preparation-surgery-antibiotics- early postoperative feeding group （early feeding group）, and bowel preparation-surgery-antibiotics- postoperative fasting group. The rats were sacrificed and stool specimens were collected from the cecum. Stools were diluted and transferred to selective medium. Bacteria counts were calculated after 48 hours of culture under constant temperature. The changes in gut flora between the different groups were compared in terms of E.coli, Bacteroides, Bzifidobacterium, and Enterococcus. Results Compared with the control group, the total bacteria, Bacteroid, Enterococcus, Bifidobacterium were all significantly decreased （P〈0.05）, while the E.coli count and the bacillus/coccus ratio were significantly elvevated （P〈0.05）. In the bowel preparation group, the total bacteria count, Bacteroid, Enterococcus, Bifidobacterium were all significantly decreased（P〈0.05）, while the E.coli count remained stalble（P〉0.05） and the bacillus/coccus ratio was significantly elvevated （P〈0.05）. In the fasting group, the total bacteria count, Bacteroid, Enterococcus, Bifidobacterium were all significantly decreased （P〈0.05）, while the E.coli count remained stalble （P〉0.05） and the bacillus/coccus ratio was significantly elvevated（P〈0.05）. Early postoperative feeding increased E.coli, Enterococcus, and total bacteria count （P〈0.05）, and lowered bacillus/coccus ratio （P〈0.05） as compared to the fasting group. Conclusions Antibiotics, bowel preparation, and fasting have influence on the gut flora of SD rats in count and bacillus/coccus ratio, leading to dysbiosis. Early postoperative feeding may improve

关 键 词：肠道菌群 菌群失调 抗生素 肠道准备 禁食 

分 类 号：R735[医药卫生—肿瘤]