原发性纵隔B细胞淋巴瘤与非纵隔弥漫性大B细胞淋巴瘤在基因重排检测与EB病毒感染方面的对比研究  

Comparative study between primary mediastinal B-cell lymphoma and non-mediastinal diffuse large B-cell lymphoma by immunoglobulin gene rearrangement and Epstein-Barr virus infection detection

作　　者：钟定荣[1] 凌庆[1] 师晓华[1] 梁智勇[1] 刘彤华[1] 

机构地区：[1]中国医学科学院北京协和医学院北京协和医院病理科,100730

出　　处：《中华病理学杂志》2012年第6期361-365,共5页Chinese Journal of Pathology

摘　　要：目的探讨原发性纵隔B细胞淋巴瘤（PMBCL）与非纵隔弥漫性大B细胞淋巴瘤（DLBCL）在基因重排检测与EB病毒感染方面的差异。方法收集2000年9月至2011年5月北京协和医院手术切除的PMBCL20例，选取同期非纵隔DLBCL30例作为对照，进行回顾性病理学分析并进行重链和轻链基因重排检测、EBER原位杂交检测。结果20例PMBCL中6例基因重排检测呈单克隆性，均为弱阳性条带；30例DLBCL中27例基因重排检测呈单克隆性（90．0％），均为强阳性条带；EBER在20例PMBCL中检测到1例阳性，在30例DLBCL中检测到2例阳性。结论PMBCL在重链和轻链的基因重排检测方面单克隆率低，而非纵隔DLBCL单克隆率高，二者具有显著差异；但原位杂交检测EBER，二者感染率均低，无明显区别。Objective To investigate the differences between primary mediastinal B-cell lymphoma （PMBCL） and non-mediastinal conventional diffuse large B-cell common lymphoma （DLBCL） in immunoglobulin gene rearrangement and EB virus infections. Methods Twenty cases of PMBCL and 30 cases of non-mediastinal DLBCL were collected from September, 2000 to May, 2011. Pathological data were retrospectively analysed. Immunoglobulin heavy chain and light chain gene rearrangements and EBER in-situ hybridization were performed. Results Six of 20 cases of PMBCL showed monoclonal gene rearrangement, all of which were weakly detected. Twenty-seven of 30 cases of ordinary diffuse large B-cell lymphoma showed monoelonal gene rean＇angement, which were strongly detected （90. 0% ）. Only 1 of 20 cases PMBCL and 2 of 30 cases of DLBCL were positive for EBER in-situ hybridization. Conclusions The detection rate of immunoglobulin gene rearrangement is significantly lower in PMBCL than that of non-mediastinal DLBCL. However, EB virus infection rates are very low in both types of lymphomas.

关 键 词：淋巴瘤 B细胞 淋巴瘤 大细胞 弥漫型 纵隔肿瘤 基因重排 

分 类 号：R734[医药卫生—肿瘤]