山东地区肾上腺素能β3受体基因多态性与冠心病的相关性研究  

Relationship between β3-Adronergenil Receptor Genetic Polymorphism and Risk Factors of CHD Patients in ShanDong Region

作　　者：崔金兴[1] 崔国方[2] 钟志欢[3] 张积涛[2] 陈惠[2] 

机构地区：[1]青岛大学医学院,山东青岛266033 [2]青岛大学医学院附属海慈医疗集团心内科,山东青岛266033 [3]青岛海慈医疗集团特需保健科,山东青岛266033

出　　处：《现代生物医学进展》2012年第11期2141-2144,共4页Progress in Modern Biomedicine

摘　　要：目的:研究山东地区肾上腺素能β3受体(β3-AR)基因多态性与冠心病及其危险因素的之间的关系。方法:对相互间无一级亲属关系的120例冠心病患者及114例非冠心病正常对照组人群,进行体重指数、腰臀围比、空腹血糖、血脂、血压、心电图、心脏彩超等检测,同时应用聚合酶链反应-限制性片段长度多态性(PCR-RFLP)核苷酸分型技术检测肾上腺素能β3受体基因及其基因多态性进行分析。结果:(1)冠心病组和正常对照组Trp64Trp、Trp64Arg和Arg64Arg的基因型频率分别为68.3%,30.0%,1.6%和69.2%,29.8%,0.9%。冠状动脉粥样硬化性心脏病组Arg等位基因频率(16.6%)与对照组(15.8%)频率近似(P>0.05),突变频率在冠心病与非冠心病之间相比无显著性差异。(2)多态性分布在冠状动脉粥样硬化性心脏病组不同冠脉病变支数亚组中也无显著性差异(x2=0.471,P=0.790)。结论:肾上腺素能β3受体基因Trp64Arg突变与山东地区人群冠心病的发生、发展无明显相关性。Objective： To investigate the Relationship between β3-adronergenil receptor genetic polymorphism and risk factors of patients with coronary heart disease（CHD）in Shandong region.Methods： The 120 patients with coronary heart disease and 114 healthy controls were involved in the study.These β3-adronergenil receptor genetic polymorphisms were analyzed by PCR-restriction fragment length polymorphism（PCR-PFLP）.The indexes such as body weight,height,Body-mass index（BMI）,waist circumstance,hip circum-stance,blood pressure,lipids,fast plasma glucose and electrocardiogram were detected in all subjects.Results：（1） The frequen-cies of Trp/Trp,Trp/Arg andArg/Arg were 68.3%,30.0%,1.6% in the CHD group while those were 69.2%,29.8%And 0.9% in the control group.The frequency of Arg allele were 16.6% in the CHD group and 15.8% in the control group（P0.05）.（2） The frequencies of Trp/Arg and Arg/Arg genotypes of the Trp64Arg polymorphism were not statistically different among CHD patients with one,two and three or more significantly diseased vessels（x2=0.471,P=0.790）.Conclusion： The findings suggest no significant association was found between β3-AR Trp64Arg polymorphism and CHD in ShanDong region.

关 键 词：冠心病 肾上腺素能β3受体 基因突变 

分 类 号：R541.1[医药卫生—心血管疾病]