提高重组质粒转化E. coli DH5α方法的研究  

Study on the Method of Transforming Recombinant Plasmid DNA into E. coli DH5α

作　　者：李晶[1] 

机构地区：[1]黑龙江生物科技职业学院食品生物系,黑龙江哈尔滨150025

出　　处：《安徽农学通报》2012年第9期54-55,共2页Anhui Agricultural Science Bulletin

摘　　要：研究了质粒载体和外源DNA的浓度、酶切体系的酶切时间以及酶切产物的链接时间对转化效率的影响。结果表明,当pUC19质粒载体的浓度为25ng/μL,外源基因λDNA的浓度为100ng/μL,酶切时间3h,且连接时间为14h时,构建出的重组质粒转化大肠杆菌DH5α效率最高。Effect of plasmid vector and exogenous DNA concentration, digestion time and connection time of digestion prod- ucts on the transformation efficiency is studied. It shows that the highest transformation efficiency into E. coli DH5α of the constructed recombinant plasmid can be achieved when plasmid vector PUC19 concentration is 25ng/μL, exogenous gene XDNA concentration is 100ng/μL, digestion time is 3h and connection time is 14h.

关 键 词：大肠杆菌DH5α菌株 重组质粒 转化效率 

分 类 号：Q939.121[生物学—微生物学]