14种红树植物对动物病原菌的抑菌活性  

Inhibitory activity of fourteen mangrove plants against animal pathogens

作　　者：邓业成[1] 骆海玉[1] 张丽珍[1] 秦卉[1] 廖永梅[1] 李瑞钰[1] 

机构地区：[1]广西师范大学生命科学学院,珍稀濒危动植物生态与环境保护省部共建教育部重点实验室,广西桂林541004

出　　处：《海洋科学》2012年第3期37-41,共5页Marine Sciences

基　　金：广西自然科学基金项目(桂科自0991097);珍稀濒危动植物生态与环境保护省部共建教育部重点实验室科研项目(桂科能1001Z014)

摘　　要：采用带药培养基涂布法测定了14种红树植物甲醇提取物及从中分离获得的2种纯化合物对大肠杆菌(Escherichia coli)、绿脓杆菌(Pseudomonas aeruginosa)、伤寒沙门氏菌(Salmonella typhi)、痢疾志贺氏菌(Shigella dysenteriae)、普通变形杆菌(Proteus vulgaris)、金黄色葡萄球菌(Staphyloccocus aureus)、藤黄微球菌(Micrococcus luteus)、溶壁微球菌(Micrococcus lysodeikticus)和蜡样芽胞杆菌(Bacillus cer-eus)9种动物病原菌的抑菌活性。结果表明,苦郎树(Clerodendrum inerme)、海漆(Excoecaria agallocha)、白骨壤(Avicennia marina)、苦槛蓝(Myoporum bontioides)、银叶树(Heritiera littoralis)和榄李(Lumnitzeraracemosa)有比较广谱的抗菌活性。苦槛蓝和海漆的抑菌活性较高,最低抑制浓度(Minimum inhibitoryconcentration,MIC)值分别为1～4 g/L和2～6 g/L。从苦郎树中分离获得的纯化合物KLS-46对伤寒沙门氏菌、金黄色葡萄球菌、溶壁微球菌和蜡样芽胞杆菌的MIC值分别为0.1、0.05、0.1和0.05 g/L,对其余5种病原菌的MIC值均大于0.4 g/L。从病原菌对红树植物的敏感性看,藤黄微球菌和蜡样芽胞杆菌对红树植物比较敏感。The inhibitory activity of methanol extracts from fourteen mangrove plants and two isolated compounds against Escherichia coli, Pseudomonas aeruginosa, Salmonella typhi, Shigella dysenteriae, Proteus vulgaris, Staphyloccocus aureus, Micrococcus luteus, Micrococcus lysodeikticus and Bacillus cereus was determined using plate culture medium coating method. The results showed that the extracts of Clerodendrum inerme, Excoecaria agallocha, Avicennia marina, Myoporum bontioides, Heritiera littoralis and Lumnitzera racemosa exhibited broad-spectrum antibacteria activity. The inhibitory activity of M. bontioides and E. agallocha was relatively high and the MIC value was 1-4 and 2-6 g/L, respectively. The KLS-46 purified from C. inerme had the MIC values of 0.1, 0.05, 0.1 and 0.05 g/L against S. typhi, S. aureus, M. lysodeikticus and B. cereus, respectively, but showed almost inactive against the other five pathogens with MIC value more than 0.4 g/L. The 34. luteus and B. cereus were more sensitive to the extracts from mangrove plants.

关 键 词：红树植物 动物病原菌 抑菌活性 

分 类 号：S435.72[农业科学—农业昆虫与害虫防治]