ATC-IC致敏的树突状细胞诱导T淋巴细胞抗肿瘤效应的研究  

Anti-tumor Effects of T Lymphocytes Induced by Dendritic Cells Pulsed with ATC-IC

作　　者：孙海燕[1] 刘晓辉[1] 杨明花[1] 马楠[1] 刘云鹏[2] 姜又红[1] 

机构地区：[1]中国医科大学附属第一医院肿瘤研究所第二研究室,沈阳110001 [2]中国医科大学附属第一医院肿瘤内科,沈阳110001

出　　处：《中国医科大学学报》2012年第5期409-412,426,共5页Journal of China Medical University

基　　金：辽宁省医学高峰建设工程专项(2010017)

摘　　要：目的探讨凋亡的肾癌细胞与G250单克隆抗体(mAb)形成的复合物(ATC-IC)致敏树突状细胞(DC)后,DC对T淋巴细胞的激活、增殖作用及对肾透明细胞癌细胞的抑癌效应。方法制备凋亡的肾癌细胞与G250 mAb形成的复合物ATC-IC;分离健康供血者外周血单个核细胞及T淋巴细胞,联合应用粒—巨细胞集落刺激因子及白细胞介素从单个核细胞中培养出DC,以制备的ATC-IC刺激DC为实验组,以凋亡的肾癌细胞刺激DC和未经任何因素刺激的DC为对照组,检测各组DC对T淋巴细胞的细胞增殖动力学的影响,并用CCK-8测定诱导的细胞毒性T淋巴细胞对肾癌细胞株786-0和肺癌细胞株A549的杀伤活性。结果 ATC-IC致敏的DC诱导T淋巴细胞强烈的增殖反应,与对照组比较具有统计学差异(P<0.01);增殖后的T淋巴细胞对786-0的杀伤率明显高于对照组(P<0.05),而2组对A549细胞均无明显杀伤活性。结论经ATC-IC致敏的DC能诱导T淋巴细胞增殖,在体外可有效的诱导特异性抗肾癌效应。Objective To investigate the activation and proliferation of cytotoxic T lymphocytes（CTL） induced by dendritic cells pulsed with the complexes ATC-IC and their anti-tumor effects on renal carcinoma cells.Methods The complexes ATC-IC were prepared with apoptotic renal carcinoma cells and G250mAb.Monocytes and T lymphocytes were isolated from the peripheral blood in healthy subjects.DCs were cultivated in monocytes by granulocyte-macrophage colony and interleukin.The DCs stimulated by ATC-IC were experimental group,while the DCs loaded with ATC and without any stimulation were control group.The effects of the DCs in each group on CTL were tested.The effects of CTL on renal carcinoma cells（786-0） and lung cancer cells（A549） were tested by CCK-8 assays.Results Compared with the control group,the proliferation of CTL induced by DC pulsed with the complexes ATC-IC was statistically significant（P 〈 0.01）.Compared with the control group,proliferated CTL had higher cytotoxicity against 786-0 cells（P 〈0.05）.Two groups had no significant killing activity on A549 cells.Conclusion DCs pulsed by ATC-IC could induce a significant T lymphocyte proliferation and the proliferated CTL could induce specific anti-kidney tumor effects in vitro.

关 键 词：树突状细胞 ATC-IC T淋巴细胞 细胞毒性 

分 类 号：R737.11[医药卫生—肿瘤]