卵泡刺激素β基因编码区SNPs与糯谷猪繁殖性能的关联性分析  

Association Analyses of Single Nucleotide Polymorphism of FSHβ Gene on Reproductive Performance in Nuogu Pig

作　　者：褚维伟[1] 高颖[2] 林家栋[1] 闫雷[1] 潘兰兵[1] 李沛丽[1] 

机构地区：[1]贵州大学动物科学学院,高原山地动物遗传育种与繁殖省部共建教育部重点实验室,贵州贵阳550025 [2]贵州大学动物科学学院,贵州省动物疫病研究室,贵州贵阳550025

出　　处：《中国畜牧兽医》2012年第5期148-151,共4页China Animal Husbandry & Veterinary Medicine

基　　金：贵州省农业攻关项目"糯谷猪种质资源保护;开发利用与产业化配套技术研究"(黔科合NY字(2008)3042)

摘　　要：本试验采用RCR-SSCP及测序的方法检测了58头糯谷猪母猪卵泡刺激素(follicle-stimulating hormone,FSH)β基因编码区3个外显子的多态性,并分析了不同基因型与繁殖性能的关联性,结果发现,在外显子1和外显子3中未发现多态位点,外显子2中发现了2个新的多态位点,共检测出3个等位基因,5个基因型,未检测到BB基因型。其中A等位基因在6699位点发生了C→T突变,B等位基因在6708位点发生了T→C突变,C等位基因与公布序列一致,A、B、C等位基因频率分别为0.288、0.119、0.593。2个突变均为同义突变,各基因型间的繁殖性能差异不显著。This study aimed to investigate molecular genetic characteristics of the follicle-stimulating hormone （FSH） β gene and elucidate the effects of single nucleotide polymorphisms （SNPs） of FSHβ on some reproductive performance in Nuogu pig. We used polymerase chain reaction single-strand conformation polymorphism （PCR-SSCP） and sequencing the coding sequence of the FSHβ gene in 58 individuals. The results showed that two new polymorphic sites were found in exon 2 on 6699 bp and 6708 bp. The A allele frequency was 0. 288,the B allele frequency was 0. 119,while the C allele frequency was 0. 593. There were no significant difference in five genotypes（AA,CC, AB, AC, BC）, because of the two mutant sites were the synony- my mutations.

关 键 词：糯谷猪 FSHΒ基因 外显子 PCR-SSCP SNPS 

分 类 号：S813[农业科学—畜牧学]