苍耳七提取物的体外抗氧化活性研究(英文)  

In vitro antioxidant activity of Parnassia wightianaW.extracts

作　　者：温新宝[1] 苗芳[1] 周乐[2] 张淼[1] 何全磊[1] 

机构地区：[1]西北农林科技大学生命科学学院,杨凌712100 [2]西北农林科技大学理学院,杨凌712100

出　　处：《中国天然药物》2012年第3期190-195,共6页

基　　金：supported by the National Natural Science Foundation of China (Nos. 31000865, 30771454,31172365,31170366)~~

摘　　要：目的:了解苍耳七不同提取物中总黄酮含量、抗氧化活性及黄酮含量与抗氧化活性之间的相关性。方法:用三种不同有机溶剂依次对苍耳七 50 %乙醇提取物进行萃取,分别得到氯仿提取物(CE)、乙酸乙酯提取物(EAE)、正丁醇提取物(BE)和水相提取物(WE)。总黄酮含量采用 NaNO2- Al (NO3)3-NaOH 显色法测定。抗氧化活性采用羟基自由基清除试验、超氧自由基清除试验、DPPH 自由基清除试验、还原力测定和金属离子螯合试验进行综合评价,以 BHT(2, 6-二叔丁基对甲酚)为参考物质。结果:EAE 的黄酮含量最高,其次分别为 BE, WE 和 CE。WE 和 BE 的抗氧化活性强于 CE 和 EAE. 每种提取物的抗氧化活性都显示出浓度依赖性和黄酮含量的相关性。结论:苍耳七提取物可以被用作天然抗氧剂。AIM：To investigate the total flavonoid contents and in vitro antioxidant activity of different extracts from Parnassiawightiana and their correlation.METHODS：50% ethanol（in water,V/V） extract from Parnassia wightiana W.was successivelyextracted by using 3 kinds of different solvents to provide chloroform extract（CE）,ethyl acetate extract（EAE）,n-butanol extract（BE）and water extract（WE）.The content of flavonoids in each extract was determined using the NaNO2-Al（NO3）3-NaOH method.Differentantioxidant assays（OH？ O2-？ DPPH？scavenging activity,reducing power and metal chelating activity） were employed to evaluate theantioxidant activities of four extracts,using butylated hydroxytoluene（BHT） as a reference.RESULTS：The total flavonoid contentin EAE was the highest,followed BE,WE and CE.WE and BE showed better antioxidant activity than CE and EAE.Each extractshowed concentration dependent antioxidant activity and a high correlation between content of flavonoids and antioxidant activity.CONCLUSION：The extrat of Parnassia wightiana W.may be used as natural antioxidant.

关 键 词：苍耳七 提取物 黄酮类化合物 自由基 抗氧化活性 

分 类 号：R965[医药卫生—药理学]