条斑紫菜藻红蛋白粗提方法比较  

Comparison of crude extraction methods of phycoerythrin from Porphyra yezoensis

作　　者：蔡春尔[1] 李春霞[1] 藤一悦[1] 林子龙[1] 顾碧莹[1] 何培民[1] 

机构地区：[1]上海海洋大学水产与生命学院,上海201306

出　　处：《上海海洋大学学报》2012年第3期368-373,共6页Journal of Shanghai Ocean University

基　　金：国家高技术研究发展项目(2007AA09Z406);国家科技支撑计划课题(2012BAC07B03);上海市教育委员会重点学科建设项目(S30701)

摘　　要：研究了我国主要经济红藻之一条斑紫菜藻红蛋白各种粗提方法。采用"溶胀+组织捣碎"法破碎条斑紫菜叶状体细胞,依次研究活性炭、利凡诺等化学试剂和等电点、溶液浓度、pH、温度、差速离心等物理条件对条斑紫菜破碎液中藻红蛋白纯度和产率的影响。结果显示:小量活性碳与利凡诺的结合使用不能提高藻红蛋白纯度,单独使用活性碳,随用量提高,其纯度上升,在活性碳用量是紫菜用量的1/10时提纯效果最佳。藻红蛋白等电点沉淀后纯度反而下降,但pH调节与硫酸铵沉淀结合可显著提升纯度,且与原溶液浓度成正比,藻红蛋白纯度最高达到1.35。此外,盐析过程中差速离心还可继续提升纯度,达到1.42,而通过控制温度来变性蛋白的方法不能达到提纯的效果。实验为进一步改进优化条斑紫菜藻红蛋白粗提做了铺垫。We compared the methods of crude phycoerythrin extraction from Porphyra yezoensis, a main economic red alga in China. Porphyra yezoensis thallus was broken with ＂swelling ＆ smash＂ method, and crude extract was dealed in sequence with chemical reagent such as activated carbon, rivanol or physical condition such as isoelectric point, concentration, pH, temperature and differential centrifugation. It showed that the purity of phycoerythrin increased when activated carbon was heavily applied alone. When usage of activated carbon achieved two percent of that of Porphyra, the effect was best. And this method was much better than combined using of activated carbon and rivanol on a small scale. On the other hand, ammonium sulfate precipitation combined with pH regulating increased the purity, which was in line with concentration of solution and arrived at topmost purity of 1.35, which achieved 1.42 after differential centrifugation followed. While isoelectric precipitation or temperature control decreased the purity. This research laid the groundwork for preparation of phycoerythrin from Porphyra yezoensis.

关 键 词：条斑紫菜 藻红蛋白 活性碳 利凡诺 差速离心 

分 类 号：Q819[生物学—生物工程] S968.43[农业科学—水产养殖]