野葛及药材生物学特性的初步研究  

Study on Biological Character of Pueraria lobata and Medicinal Materials

作　　者：纪宝玉[1] 裴莉昕[1] 陈随清[1] 董诚明[1] 

机构地区：[1]河南中医学院药学院,郑州450008

出　　处：《中国实验方剂学杂志》2012年第10期108-112,共5页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：科技部"十一五"科技支撑计划项目(2006BAI06A10)

摘　　要：目的:解野葛生长习性和葛根活性物质含量的动态变化。方法:以实地调查的方式观测野葛的生长发育情况,《中国药典》方法测定醇溶性浸出物的含量,高效液相法测定葛根中葛根素、大豆苷、大豆苷元的含量,紫外分光光度法测定总黄酮含量,苯酚-硫酸法测定葛根多糖含量。结果:初步揭示了野葛的生长发育情况;发现葛根中各活性物质含量变化有一定规律,1～12月份中以8,9月份各活性物质含量最高,不同年限葛根各活性物质含量随年限增长而增高。结论:研究了野葛生长发育过程与葛根各活性物质积累之间的关系和机制,为野葛的采收、合理开发利用提供一定的科学依据。Objective： Studies on growth habit of Pueraria lobata and dynamic changes of the active substance content of puerariae Lobatae Radix. Method： The method of determining the content of alcohol-soluble chemical composition was in line with PharmacQpeia; puerarin, contents of flavonoids were assayed by ultraviolet spectroscopy. aidzin, daidzein were determinated by HPLC. The The contents of polysaccharide were determined by phenol-sulfuric acid method. Result： This paper elementarily reveals growth habit of Pueraria lobata： The content of active substance appeared regular changes and was highest in August and September of perennial Puerariae Lobatae Radix. Conclusion： This result fruther provides scientific basis for resoures protection, rational development and comprehensive of P. lobata.

关 键 词：野葛 生物学特性 生长习性 有效成分 

分 类 号：R282[医药卫生—中药学]