免疫亲和柱净化HPLC柱后光化学衍生化法检测中药及染菌中药制剂中间体的黄曲霉毒素  

Investigation on Detection of Aflatoxins in Chinese Materia Medica and Pharmaceutical Intermediates Microbiological Contaminated by Immunoaffinity Column Clean-up Combined with Post-column Derivatization

作　　者：胡一晨[1] 万丽[1] 范成杰[1] 吕维[1] 杨芳[1] 吉琅[1] 

机构地区：[1]成都中医药大学药学院,中药材标准化教育部重点实验室,四川省中药资源系统研究与开发利用省部共建国家重点实验室培育基地,中央与地方共建中药安全性评价实验室,成都611137

出　　处：《中国实验方剂学杂志》2012年第10期116-119,共4页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：四川省教育厅科技创新重大培育项目(09ZZ08)

摘　　要：目的:采用免疫亲和柱净化,结合柱后光化学衍生化的高效液相色谱-荧光检测器建立同步检测常用中药材及染菌中药制剂中间体中的黄曲霉毒素B1,B2,G1,G2的方法。方法:采用免疫亲和柱净化洗脱结合柱后光化学衍生手段,建立黄曲霉毒素B1,B2,G1,G2的分析方法,并分别对14种中药材及染菌中药制剂中间体进行检测。结果:黄曲霉毒素B2和G2,B1和G1分别在0.15～6.0和0.5～20.0 ng·mL-1线性关系良好,方法准确稳定。所选的14种中药中川芎和柏子仁检测出黄曲霉毒素B1,而以染菌中药川芎所制备的5种提取物中均未检出黄曲霉毒素。结论:采用此方法检测常用中药材及其制剂中间体中的黄曲霉毒素无干扰性杂峰,结果准确可靠。Objective： To establish a method to determine aflatoxins BI, B2, G1, G2 in Chinese Materia Medica and pharmaceutical intermediates microbiological contaminated by immunoaffinity column clean-up combined with post-column derivatization and HPLC-FD detection. Method： Using immunoaffinity column clean- up combined with post-column derivatization, the analytical method to detect aflatoxins B1, B2, G1, G2 was established firstly, and then 14 kind of Chinese Materia Medica and pharmaceutical intermediates microbiological contaminated was detected respectively. Result： The method with the great linear concentration range of 0. 15-6.0 ng·mL^-1 for aflatoxins G2, B2, and 0.5-20.0 μg· L^-1for G1, B1 respectively, was stable and accurate. As a result, aflatoxins Blwas detected in Chuanxiong Rhizoma and Platycladi Semen in the 14 kind of Chinese Materia Medica, while there were none of aflatoxins in the pharmaceutical intermediates made by Chuanxiong Rhizoma. Conclusion： The method established in this study was specific and accurate for the detection of aflatoxins in Chinese Materia Mediea and pharmaceutical intermediates.

关 键 词：提取物 黄曲霉毒素 免疫亲和柱 光化学衍生 

分 类 号：R284.1[医药卫生—中药学]