逆转录病毒介导IL-2cDNA治疗小鼠淋巴瘤的研究  

A Research on IL-2 Gene Therapy for Mice Lymphoma Mediated by Retroviral Vector

作　　者：鲁晓萱[1] 曹荣月[2] 苏宁[3] 张晓明[3] 蒋清[1] 单祥年[1] 

机构地区：[1]南京铁道医学院生物学教研室,江苏南京210009 [2]中国药科大学生物化学教研室,江苏南京210009 [3]南京铁道医学院病理学教研室,江苏南京210009

出　　处：《南京铁道医学院学报》2000年第1期1-4,共4页Journal of Nanjing Railway Medical College

基　　金：国家自然科学基金资助项目!(39370375)

摘　　要：目的 :研究人白细胞介素 2 (hIL 2 )转基因小鼠淋巴瘤体内成瘤性。方法 :体外将hIL 2基因转入小鼠淋巴瘤p3 88细胞。用PCR、RT PCR、dotblot法检测hIL 2基因在细胞内的整合及表达 ,四甲基偶氮唑盐比色 (MTT)法检测转导细胞hIL 2分泌。体内试验将动物分为A9组、对照组和体内转染治疗组 3组。 17d后处死所有动物 ,观察肿瘤生长状况。结果 :hIL 2基因已成功地整合到小鼠p3 88细胞基因组内。p3 88/IL 2细胞分泌的hIL 2量为 3 7.4U·ml-1。A9组和体内转染治疗组动物肿瘤体积明显减小 ,与对照组肿瘤体积有显著性差异 (P <0 .0 5 )。结论 :hIL 2基因经体内或体外法转入p3 88/ILObjective The experiment was designed to study the tumorigenesis of human interleukin?2(hIL?2) transgenetic mice lymphoma.Methods The hIL?2 gene was transferred into mice lymphoma cell line in vitro . The DNA and RNA of hIL?2 transduced p388 cell were analyzed by PCR,RT?PCR,dot?blot.The secretion of hIL?2 was measured by 3?(4,5?dimethylthiazole?2?yl)?2,5?diphenyl?tetrazolium bromide(MTT) method.The experimental animals were invided into 3 groups.Group 1 was treated with A9 cells,group 2 served as control and group 3 was treated with p338?1 cells and A8 supernatant.The size and weight of tumor were measured at day 17 after the treatment.Results The hIL2 cDNA had been inserted into the genome of p388/IL?2 cell and had the expression of hIL2 mRNA, p388/IL?2 cell would secret 37.4?U·ml -1 hIL?2. The tumor size in group 1 and 3 reduced markedly and significantly differed from that in control group( P <0.05).Conclusion The tumorigensis of p388/IL2 cell,which was transduced by hIL2 gene in vivo or in vitro ,was reduced in mice.

关 键 词：逆转录病毒载体 基因治疗 淋巴瘤 HIL-2 

分 类 号：R733.05[医药卫生—肿瘤]